Caveolin and MAL, two protein components of internal detergent-insoluble membranes, are in distinct lipid microenvironments in MDCK cells

Biochemical and Biophysical Research Communications
J MillánM A Alonso

Abstract

The MAL proteolipid and caveolin have been identified as components of internal detergent-insoluble membrane microdomains enriched in glycolipids and cholesterol. We have addressed the study of the glycolipid-enriched membranes in cells expressing endogenously only either MAL (Jurkat T cells) or caveolin (epithelial A498 cells) and in polarized MDCK cells which express both proteins simultaneously. Subcellular fractionation by centrifugation to equilibrium in sucrose gradients of Triton X-100 cell extracts from Jurkat and A498 cells revealed that MAL and caveolin are incorporated in detergent-insoluble buoyant membranes independently of the expression of each other and indicated the existence in these cells of insoluble membrane microdomains with either MAL or caveolin. Immunofluorescence analysis in MDCK cells indicated that both MAL and caveolin were located in the Golgi region, whereas caveolin was found in addition at the cell surface. Biochemical analysis in these cells revealed the existence of distinct membrane microenvironments differentially susceptible to detergent solubilization containing either internal MAL or internal plus surface caveolin. The observed heterogeneity within the internal glycolipid-enriched membran...Continue Reading

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Citations

Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Aug 17, 2012·American Journal of Physiology. Cell Physiology·Vinita TakiarMichael J Caplan
Dec 25, 2012·Molecular Biology of the Cell·Juan F ArandaJaime Millán
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Feb 26, 2020·Veterinary Research·Jonatan Dorca-ArévaloJuan Blasi
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Jan 28, 1999·Biochemical and Biophysical Research Communications·R Puertollano, M A Alonso
Sep 12, 2001·Advanced Drug Delivery Reviews·J CouetM C Drolet

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