PMID: 9525638Apr 3, 1998Paper

CBF, Myb, and Ets binding sites are important for activity of the core I element of the murine retrovirus SL3-3 in T lymphocytes

Journal of Virology
A L ZaimanJ Lenz

Abstract

Transcriptional enhancers within the long terminal repeats of murine leukemia viruses are major determinants of the pathogenic properties of these viruses. Mutations were introduced into the adjacent binding sites for three transcription factors within the enhancer of the T-cell-lymphomagenic virus SL3-3. The sites that were tested were, in 5'-to-3' order, a binding site for core binding factor (CBF) called core II, a binding site for c-Myb, a site that binds members of the Ets family of factors, and a second CBF binding site called core I. Mutation of each site individually reduced transcriptional activity in T lymphocytes. However, mutation of the Myb and core I binding sites had larger effects than mutation of the Ets or core II site. The relative effects on transcription in T cells paralleled the effects of the same mutations on viral lymphomagenicity, consistent with the idea that the role of these sequences in viral lymphomagenicity is indeed to regulate transcription in T cells. Mutations were also introduced simultaneously into multiple sites in the SL3-3 enhancer. The inhibitory effects of these mutations indicated that the transcription factor in T cells that recognizes the core I element of SL3-3, presumably CBF, nee...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H DudekE P Reddy
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B K BrightmanH Fan
Jun 1, 1983·Cell·G Khoury, P Gruss
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L DesGroseillersP Jolicoeur
Jan 1, 1994·Molecular and Cellular Biology·C Hernandez-Munain, M S Krangel
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E OgawaY Ito

❮ Previous
Next ❯

Citations

Mar 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Juan CarabanaMichael S Krangel
Apr 2, 2016·ILAR Journal·Jaquelin P DudleySusan R Ross
Jan 19, 2007·Journal of Virology·Jennifer A MertzJaquelin P Dudley
Feb 13, 2001·Journal of Virology·J A MertzJ P Dudley
Dec 6, 2000·Seminars in Cell & Developmental Biology·J C WheelerY Ito
Feb 12, 2002·Journal of Virology·Dana R BroussardJaquelin P Dudley

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.