CBL-interacting protein kinase 25 contributes to root meristem development

Journal of Experimental Botany
Mukesh Kumar MeenaDebasis Chattopadhyay

Abstract

Co-ordination of auxin and cytokinin activities determines root meristem size during post-embryonic development. Calcineurin B-like proteins (CBLs) and their interacting protein kinases (CIPKs) constitute signaling modules that relay calcium signals. Here we report that CIPK25 is involved in regulating the root meristem size. Arabidopsis plants lacking CIPK25 expression displayed a short root phenotype and a slower root growth rate with fewer meristem cells. This phenotype was rescued by restoration of CIPK25 expression. CIPK25 interacted with CBL4 and -5, and displayed strong gene expression in the flower and root, except in the cell proliferation domain in the root apical meristem. Its expression in the root was positively and negatively regulated by auxin and cytokinin, respectively. The cipk25 T-DNA insertion line was compromised in auxin transport and auxin-responsive promoter activity. The cipk25 mutant line showed altered expression of auxin efflux carriers (PIN1 and PIN2) and an Aux/IAA family gene SHY2. Decreased PIN1 and PIN2 expression in the cipk25 mutant line was completely restored when combined with a SHY2 loss-of-function mutation, resulting in recovery of root growth. SHY2 and PIN1 expression was partially regu...Continue Reading

References

Dec 8, 1998·The EMBO Journal·A MüllerK Palme
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Mar 9, 2002·The Plant Cell·Qing TianJason W Reed
May 23, 2002·Journal of Plant Growth Regulation·Jeremiah M FasanoSimon Gilroy
May 25, 2002·The Journal of Biological Chemistry·Deming GongJian-Kang Zhu
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Quan-Sheng QiuJian-Kang Zhu
Aug 29, 2002·The Journal of Biological Chemistry·Deming GongJian-Kang Zhu
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Dec 12, 2003·Developmental Cell·Ranjan Swarup, Malcolm Bennett
May 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Anders NordströmGöran Sandberg
Apr 5, 2005·Nature Genetics·Markus SchmidJan U Lohmann
May 27, 2005·Nature·Stefan Kepinski, Ottoline Leyser
Sep 1, 1989·Plant Physiology·L EliassonE Bolander
Oct 3, 2006·Cellular and Molecular Life Sciences : CMLS·H TanakaJ Friml
Mar 17, 2007·Current Biology : CB·Raffaele Dello IoioSabrina Sabatini
Aug 2, 2007·The Plant Journal : for Cell and Molecular Biology·Brook K NelsonAndreas Nebenführ
Feb 12, 2008·Trends in Plant Science·Jennifer P C To, Joseph J Kieber
Jul 18, 2008·Annual Review of Cell and Developmental Biology·Keithanne Mockaitis, Mark Estelle
Dec 5, 2008·Trends in Plant Science·Sheng Luan
Feb 4, 2009·The Plant Journal : for Cell and Molecular Biology·Vineeta TripathiDebasis Chattopadhyay
Jul 28, 2009·Development·Jan Petrásek, Jirí Friml
Oct 17, 2009·The Plant Journal : for Cell and Molecular Biology·Oliver BatisticJörg Kudla
Jul 8, 2010·Current Biology : CB·Laila MoubayidinSabrina Sabatini
Aug 25, 2010·Methods in Molecular Biology·Serena Perilli, Sabrina Sabatini
May 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sumiko AdachiMasaaki Umeda

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Citations

Mar 15, 2019·Journal of Experimental Botany·Muhammed Jamsheer KAshverya Laxmi
Apr 9, 2020·Plant Signaling & Behavior·Xiao Pan ZhangFu Shun Hao
Aug 14, 2020·International Journal of Molecular Sciences·Xiao MaZhen-Hui Gong
Aug 21, 2021·Plant Cell Reports·Pooja VermaGirdhar K Pandey

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Methods Mentioned

BETA
genotyping
PCR
two-hybrid
Y2H
light microscopy
electrophoresis
Fluorescence
transgenic
confocal microscopy
myristoylation

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SigmaStat
ImageJ

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