CCA addition to tRNA: implications for tRNA quality control.

IUBMB Life
Ya-Ming Hou

Abstract

The CCA sequence is conserved at the 3' end of all mature tRNA molecules to function as the site of amino acid attachment. This sequence is acquired and maintained by stepwise nucleotide addition by the ubiquitous CCA enzyme, which is an unusual RNA polymerase that does not use a nucleic acid template for nucleotide addition. Crystal structural work has divided CCA enzymes into two structurally distinct classes, which differ in the mechanism of template-independent nucleotide selection. Recent kinetic work of the class II E. coli CCA enzyme has demonstrated a rapid and uniform rate constant for the chemistry of nucleotide addition at each step of CCA synthesis, although the enzyme uses different determinants to control the rate of each step. Importantly, the kinetic work reveals that, at each step of CCA synthesis, E. coli CCA enzyme has an innate ability to discriminate against tRNA backbone damage. This discrimination suggests the possibility of a previously unrecognized quality control mechanism that would prevent damaged tRNA from CCA maturation and from entering the ribosome machinery of protein synthesis. This quality control is relevant to cellular stress conditions that damage tRNA backbone and predicts a role of CCA ad...Continue Reading

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M P Deutscher, N B Reuven
Jan 24, 1998·Nature·T A Steitz
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·Z LiM P Deutscher
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·E Hajnsdorf, P Régnier
Jul 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·K TomitaH Masaki
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·K Tamura, P Schimmel
Feb 28, 2002·The EMBO Journal·Zhongwei LiMurray P Deutscher
Apr 3, 2002·Biochemistry·Malini SethYa-Ming Hou
Apr 12, 2002·The Journal of Biological Chemistry·Zhuan-Fen Cheng, Murray P Deutscher
Mar 7, 2003·The Journal of Biological Chemistry·Yukihide TomariTakuya Ueda
May 6, 2003·Journal of Molecular Biology·Martin A AugustinClemens Steegborn
May 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhuan-Fen Cheng, Murray P Deutscher
Aug 16, 2003·Developmental Cell·Alexei A AravinThomas Tuschl
Aug 28, 2003·The Journal of Biological Chemistry·Murray P Deutscher
Nov 19, 2003·Molecular Microbiology·Fátima CairrãoCecília Maria Arraiano
Apr 6, 2004·Current Opinion in Microbiology·Gisela StorzAixia Zhang
Aug 6, 2004·Nature·Kozo TomitaOsamu Nureki
Oct 13, 2004·Nucleic Acids Research·Louis LevingerCatherine Florentz
Oct 19, 2004·Annual Review of Microbiology·Susan Gottesman
Oct 19, 2004·Nature Structural & Molecular Biology·Scott HauensteinJohn J Perona
Nov 4, 2004·Molecular Microbiology·Bijoy K MohantySidney R Kushner
Jan 25, 2005·Molecular Cell·Zhuan-Fen Cheng, Murray P Deutscher
Oct 26, 2005·RNA·Jian LuAnders S Byström
Jan 3, 2006·Molecular Cell·Andrei AlexandrovEric M Phizicky
Feb 3, 2006·Nucleic Acids Research·Murray P Deutscher
Aug 9, 2006·The Journal of Biological Chemistry·Helen A Vincent, Murray P Deutscher
Sep 29, 2006·The Journal of Biological Chemistry·Vasanti Subramanian Anand, Smita S Patel
Mar 16, 2007·Nucleic Acids Research·Barbara Wegscheid, Roland K Hartmann
Oct 24, 2007·BMC Molecular Biology·Marion ScheibeMario Mörl
Dec 18, 2007·Nucleic Acids Research·Henry J HaiserMarie A Elliot
Jan 15, 2008·Trends in Microbiology·Primrose P E FreestoneMark Lyte

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Citations

Oct 17, 2015·FEBS Letters·Temple F Smith, Hyman Hartman
Oct 18, 2011·Journal of Molecular Biology·Takao IgarashiYa-Ming Hou
Jul 15, 2015·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Heike Betat, Mario Mörl
Jul 3, 2016·Orphanet Journal of Rare Diseases·Yehani WedatilakeShamima Rahman
Sep 19, 2016·Methods : a Companion to Methods in Enzymology·Michael H Schwartz, Tao Pan
Aug 18, 2016·The Journal of Allergy and Clinical Immunology·Glynis FransXavier Bossuyt
Jan 25, 2017·Biomolecules·Juan Campos GuillenVerónica Morales Tlalpan
Sep 16, 2018·RNA·Howard Gamper, Ya-Ming Hou
Feb 25, 2020·The EMBO Journal·Ruth L SealElspeth A Bruford
May 31, 2020·International Journal of Molecular Sciences·Angelo SladeMartin Holcik
Jul 18, 2017·Frontiers in Genetics·Tapan K Mohanta, Hanhong Bae
Mar 22, 2019·International Journal of Molecular Sciences·Marie-Theres PöhlerMario Mörl
Jul 29, 2020·International Journal of Molecular Sciences·Lieselotte ErberMario Mörl
Dec 3, 2020·International Journal of Molecular Sciences·Oliver HennigMario Mörl

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