PMID: 11914124Mar 27, 2002Paper

CCAAT/enhancer binding protein alpha uses distinct domains to prolong pituitary cells in the growth 1 and DNA synthesis phases of the cell cycle

BMC Cell Biology
Weiqun LiuFred Schaufele

Abstract

A number of transcription factors coordinate differentiation by simultaneously regulating gene expression and cell proliferation. CCAAT/enhancer binding protein alpha (C/EBPalpha) is a basic/leucine zipper transcription factor that integrates transcription with proliferation to regulate the differentiation of tissues involved in energy balance. In the pituitary, C/EBPalpha regulates the transcription of a key metabolic regulator, growth hormone. We examined the consequences of C/EBPalpha expression on proliferation of the transformed, mouse GHFT1-5 pituitary progenitor cell line. In contrast to mature pituitary cells, GHFT1-5 cells do not contain C/EBPalpha. Ectopic expression of C/EBPalpha in the progenitor cells resulted in prolongation of both growth 1 (G1) and the DNA synthesis (S) phases of the cell cycle. Transcription activation domain 1 and 2 of C/EBPalpha were required for prolongation of G1, but not of S. Some transcriptionally inactive derivatives of C/EBPalpha remained competent for G1 and S phase prolongation. C/EBPalpha deleted of its leucine zipper dimerization functions was as effective as full-length C/EBPalpha in prolonging G1 and S. We found that C/EBPalpha utilizes mechanistically distinct activities to prol...Continue Reading

References

Jan 1, 1995·Annual Review of Biochemistry·O A MacDougald, M D Lane
Aug 25, 1995·Science·N D WangG J Darlington
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·F T Lin, M D Lane
Jul 1, 1995·Immunobiology·A Wedel, H W Ziegler-Heitbrock
Apr 15, 1996·Genes & Development·R P BrunB M Spiegelman
Oct 1, 1995·Current Opinion in Genetics & Development·G J DarlingtonR W Hanson
Jul 1, 1996·Molecular and Cellular Biology·C M ConstanceR M Umek
Dec 31, 1997·Molecular and Cellular Biology·N A TimchenkoG J Darlington
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H E SorianoG J Darlington
Sep 3, 1998·The Journal of Biological Chemistry·A ZiogasG Radziwill
Oct 24, 1998·The Journal of Biological Chemistry·J Lekstrom-Himes, K G Xanthopoulos
Nov 7, 1998·The Journal of Biological Chemistry·G J DarlingtonO A MacDougald
Nov 13, 1998·The Journal of Biological Chemistry·A M Diehl
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R A RamosG L Firestone
Jan 19, 1999·Annual Review of Cell and Developmental Biology·B L Black, E N Olson
Feb 2, 1999·Experimental Cell Research·J M CraigP Vagnarelli
Feb 20, 1999·DNA and Cell Biology·H Tao, R M Umek
Mar 19, 1999·Molecular and Cellular Biology·N A TimchenkoG J Darlington
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·C MüllerA Leutz
Aug 14, 1999·Nucleic Acids Research·N A TimchenkoG J Darlington
Dec 22, 1999·Current Opinion in Cell Biology·G J Darlington
Dec 22, 1999·Current Opinion in Cell Biology·Z WuB M Spiegelman
Dec 22, 1999·Biochemical and Biophysical Research Communications·M D LaneM S Jiang
Feb 11, 2000·DNA and Cell Biology·H Tao, R M Umek
Jul 27, 2000·Molecular and Cellular Biology·B A SlomianyD T Kurtz
Aug 15, 2000·Annual Review of Nutrition·S M Rangwala, M A Lazar
Feb 24, 2001·Annals of the New York Academy of Sciences·M NordG Suske

❮ Previous
Next ❯

Citations

May 29, 2007·The Journal of Biological Chemistry·Xiaowei LiuFred Schaufele
Jun 23, 2006·Nature Methods·Ignacio A DemarcoRichard N Day
Feb 12, 2014·Cell and Tissue Research·Yuki MiyanoReiko Kurotani
Jan 18, 2003·The Journal of Biological Chemistry·Fred SchaufeleRichard N Day
Aug 16, 2006·Molecular and Cellular Biology·Ignacio A DemarcoRichard N Day
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Liang-In LinHwei-Fang Tien
Aug 18, 2010·Biophysical Journal·Yuansheng SunAmmasi Periasamy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.