PMID: 8612622Mar 1, 1996Paper

CCAAT/enhancer-binding protein beta (C/EBP beta) binds and activates while hepatocyte nuclear factor-4 (HNF-4) does not bind but represses the liver-type arginase promoter

European Journal of Biochemistry
S ChowdhuryM Takiguchi

Abstract

In an attempt to elucidate the mechanism governing liver-specific transcription of the arginase gene, we previously detected two protein-binding sites designated footprint areas A and B at positions around--90 and --55 bp, respectively, relative to the transcription start site of the rat arginase gene. Based on the finding that area A was bound by a liver-selective factor(s) related to CCAAT/enhancer-binding protein (C/EBP), we performed cotransfection assay and showed that C/EBP family members and a related factor, albumin D-element-binding protein (DBP) stimulate transcription from the arginase promoter. In addition to area A, a recombinant C/EBP beta protein bound to area B, which appeared to be primarily responsible for activation by C/EBPs. We unexpectedly found that the arginase promoter activity stimulated by C/EBPs and DBP was repressed by another liver-enriched transcription factor, hepatocyte nuclear factor-4 (HNF-4). Analysis of chimeras formed between the arginase promoter and the herpes simplex virus thymidine kinase promoter allowed us to delimit the negative HNF-4-responsive element into the region overlapping with footprint area B. However, no apparent binding of HNF-4 was observed in this negative element. We s...Continue Reading

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Citations

Oct 16, 2007·Cell and Tissue Research·Ee Hong TanNai-dy Wang
Feb 6, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eleftheria IeronymakiChristos Tsatsanis
Feb 9, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Inimary T TobyLeif D Nelin
May 13, 1999·International Journal of Experimental Pathology·M Takiguchi
Apr 17, 1997·Biochemical and Biophysical Research Communications·T GotohM Mori
Nov 14, 2006·The International Journal of Biochemistry & Cell Biology·Demetra Mavri-DamelinClare Selden

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