PMID: 15383601Sep 24, 2004Paper

CCAAT/enhancer-binding protein beta isoforms and the regulation of alpha-smooth muscle actin gene expression by IL-1 beta

The Journal of Immunology : Official Journal of the American Association of Immunologists
Biao HuSem H Phan

Abstract

The role of IL-1beta in inflammation is amply documented, but its ability to inhibit myofibroblast differentiation and, in particular, the suppression of alpha-smooth muscle actin (alpha-SMA) gene expression is less well understood. Because IL-1beta can induce C/EBPbeta expression, the role of C/EBPbeta isoforms in IL-1beta regulation of alpha-SMA gene expression was investigated in rat lung myofibroblasts. The results showed that IL-1beta inhibited alpha-SMA expression in a dose-dependent manner, which was associated with stimulation of the expression of both C/EBPbeta isoforms, liver-enriched activating protein (LAP) and liver-enriched inhibitory protein (LIP). However, a greater increase in LIP relative to LAP expression resulted in a reduced LAP/LIP ratio after IL-1beta treatment. Transfection with an LAP-expressing plasmid stimulated, whereas an LIP-expressing plasmid inhibited, alpha-SMA expression. Cells from C/EBPbeta-deficient mice had reduced levels of alpha-SMA expression and promoter activity, which failed to respond to IL-1beta treatment. Sequence analysis identified the presence of a C/EBPbeta consensus binding sequence in the alpha-SMA promoter, which, when mutated, resulted in diminished promoter activity and ab...Continue Reading

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Citations

Jul 22, 2006·American Journal of Respiratory Cell and Molecular Biology·Biao HuSem H Phan
Mar 3, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Brian HoangRobert A Edwards
Jun 19, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Goran SantakDubravko Forcić
Jun 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ning ZhangRobert P Schleimer
Apr 28, 2009·American Journal of Respiratory Cell and Molecular Biology·Biao HuSem H Phan
Mar 9, 2012·Journal of Nippon Medical School = Nippon Ika Daigaku Zasshi·Jiro UsukiAkihiko Gemma
Nov 14, 2014·The Journal of Physiological Sciences : JPS·Jamie BlackwellDavid Melzer
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May 22, 2013·Clinical & Developmental Immunology·Anna BellizziValeria Pietropaolo
Jul 27, 2007·Journal of Cellular Physiology·Matthew P Vincenti, Constance E Brinckerhoff
May 19, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Milan DodigArthur J McCullough
Dec 23, 2008·American Journal of Respiratory Cell and Molecular Biology·Liang ZhengDanielle Morse
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Eric S WhiteGalen B Toews
Nov 26, 2020·International Journal of Molecular Sciences·Elena Guillén-GómezMontserrat M Díaz-Encarnación
Dec 25, 2012·The American Journal of Pathology·Biao HuSem H Phan
May 5, 2007·Biochimica Et Biophysica Acta·Sonia Do CarmoEric Rassart
May 22, 2010·The American Journal of Pathology·Biao HuSem H Phan

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