CCAAT/enhancer-binding protein delta gene expression is mediated by autoregulation through downstream binding sites

Biochemical and Biophysical Research Communications
T YamadaM Imagawa

Abstract

CCAAT/enhancer-binding protein delta (C/EBP delta) transcription factor is sharply induced at the early stage of the acute phase response. We previously reported that the C/EBP delta gene expression is induced by the acute-phase response factor/signal transducers and activators of transcription 3 (APRF/STAT3). However, the expression level of the C/EBP delta gene is relatively high up to several hours after the stimulation, whereas APRF/STAT3 is inactivated within one hour. In this report, we identified the two C/EBP delta binding sites at the downstream region of this gene. The binding analysis revealed that both of these sites bound recombinant C/EBP delta protein. A cotransfection analysis identified these sites as the cis-elements for the autoregulation. We conclude that the C/EBP delta gene is activated by APRF/STAT3, and the expression level is then maintained by an autoregulation mechanism.

References

Jun 11, 1992·Nucleic Acids Research·M A MajérusB Bailleul
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R J ChristyM D Lane
Jul 28, 1988·Nature·P Sassone-CorsiI M Verma
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·Z ZhongJ E Darnell
Mar 1, 1995·Molecular and Cellular Biology·N TimchenkoG J Darlington
Mar 1, 1994·DNA and Cell Biology·I Berger, Y Shaul
Apr 25, 1994·Nucleic Acids Research·R BauerR Buettner
Feb 16, 1996·The Journal of Biological Chemistry·S OsadaM Imagawa
Jul 12, 1996·The Journal of Biological Chemistry·J A Spencer, R P Misra
Jun 15, 1996·The Journal of Clinical Investigation·H F ChengR C Harris
Oct 25, 1996·The Journal of Biological Chemistry·R SatoM Maeda
Apr 1, 1997·Journal of Biochemistry·T YamadaM Imagawa

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Citations

Feb 3, 2007·The Journal of Clinical Investigation·Olivier Le BacquerNahum Sonenberg
Apr 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Amarnath MaitraSarah L Gaffen
Jul 11, 2001·Biochemical and Biophysical Research Communications·P FokaD P Ramji
Apr 5, 2008·Biochemical and Biophysical Research Communications·Naomie TurgeonClaude Asselin
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·T L McCarthyM Centrella
Jun 27, 2006·The Journal of Biological Chemistry·Fang ShenSarah L Gaffen
Sep 18, 2001·The Journal of Biological Chemistry·I GheorghiuC Asselin
Nov 1, 2007·Brain : a Journal of Neurology·Adrienne MüllerDietmar Fischer
Aug 10, 2004·The Journal of Biological Chemistry·Weizhong ChangThomas L McCarthy
Sep 2, 1999·Biochemical and Biophysical Research Communications·J P O'RourkeJ DeWille

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