CCAAT/enhancer binding protein β induces motility and invasion of glioblastoma cells through transcriptional regulation of the calcium binding protein S100A4

Oncotarget
D Aguilar-MoranteA Perez-Castillo

Abstract

We have previously shown that decreased expression of CCAAT/enhancer binding protein β (C/EBPβ) inhibits the growth of glioblastoma cells and diminishes their transformation capacity and migration. In agreement with this, we showed that C/EBPβ depletion decreases the mRNA levels of different genes involved in metastasis and invasion. Among these, we found S100 calcium binding protein A4 (S100A4) to be almost undetectable in glioblastoma cells deficient in C/EBPβ. Here, we have evaluated the possible role of S100A4 in the observed effects of C/EBPβ in glioblastoma cells and the mechanism through which S100A4 levels are controlled by C/EBPβ. Our results show that C/EBPβ suppression significantly reduced the levels of S100A4 in murine GL261 and human T98G glioblastoma cells. By employing an S100A4-promoter reporter, we observed a significant induction in the transcriptional activation of the S100A4 gene by C/EBPβ. Furthermore, overexpression of S100A4 in C/EBPβ-depleted glioblastoma cells reverses the enhanced migration and motility induced by this transcription factor. Our data also point to a role of S100A4 in glioblastoma cell invasion and suggest that the C/EBPβ gene controls the invasive potential of GL261 and T98G cells thro...Continue Reading

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Citations

Mar 25, 2016·Nature Communications·Yu ChenWuhan Xiao
May 21, 2020·Cells·Malak Hassn MesratiAmir Syahir
Oct 23, 2016·Journal of Neuroinflammation·Elena Hernandez-EncinasAna Perez-Castillo
Feb 18, 2018·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Peter ArnerMikael Rydén
Feb 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Guangli LuChuning Zhang
Feb 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Laura L GonzalezMark D Turner
Aug 10, 2020·Mini Reviews in Medicinal Chemistry·Amir R AfshariAmirhossein Sahebkar

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Methods Mentioned

BETA
PCR
ChIP
immunoprecipitation
transfection

Software Mentioned

TFSEARCH
ImageJ
MatInspector

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