CCAAT/enhancer-binding protein γ is a critical regulator of IL-1β-induced IL-6 production in alveolar epithelial cells.

PloS One
Chunguang YanHongwei Gao

Abstract

CCAAT/enhancer binding protein γ (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known activation domains. C/EBPγ was originally described as an inhibitor of C/EBP transactivation potential. However, previous study demonstrates that C/EBPγ augments the C/EBPβ stimulatory activity in lipopolysaccharide induction of IL-6 promoter in a B lymphoblast cell line. These data indicate a complexing functional role for C/EBPγ in regulating gene expression. Furthermore, the expression and function of C/EBPγ during inflammation are still largely unknown. In this study, we demonstrate that C/EBPγ activation was induced by IL-1β treatment in lung epithelial cells. Importantly, we demonstrate for the first time that C/EBPγ plays a critical role in regulating IL-1β-induced IL-6 expression in both mouse primary alveolar type II epithelial cells and a lung epithelial cell line, MLE12. We further provide the evidence that C/EBPγ inhibits IL-6 expression by inhibiting C/EBPβ but not NF-κB stimulatory activity in MLE12 cells. These findings suggest that C/EBPγ is a key transcription factor that regulates the IL-6 expression in alveolar epithelial cells, and may play an important regulatory role in lung inflammatory re...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S KinoshitaT Kishimoto
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·J D BretzR C Schwartz
Apr 1, 1996·American Journal of Respiratory Cell and Molecular Biology·M CortiJ H Harrison
Aug 27, 1998·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·B Beck-SchimmerP A Ward
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·N M BlessP A Ward
Nov 1, 2001·Respiratory Research·H Fehrenbach
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ward R RiceTimothy E Weaver
Aug 15, 2002·The Journal of Biological Chemistry·Hongwei GaoRichard C Schwartz
Jun 28, 2003·American Journal of Respiratory Cell and Molecular Biology·Jeff N VanderbiltLeland G Dobbs
Feb 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C GRAHAMM J KARNOVSKY
Dec 25, 2004·American Journal of Respiratory Cell and Molecular Biology·Andrew J ThorleyTeresa D Tetley
Jan 21, 2006·Respirology : Official Journal of the Asian Pacific Society of Respirology·Robert J Mason
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongwei GaoPeter A Ward
Jun 23, 2006·European Journal of Immunology·Shibichakravarthy KannanMin Wu
Dec 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew J ThorleyTeresa D Tetley
Feb 13, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Min WuPeter G Stockley
Feb 20, 2007·Current Drug Targets. Inflammation and Allergy·Kian F Chung
Dec 19, 2007·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Jennifer M TeikenEdward C Carlson
Feb 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shibichakravarthy KannanMin Wu
Oct 15, 2009·Annual Review of Medicine·Jeffrey A WhitsettTimothy E Weaver
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Urmila MaitraLiwu Li
Apr 16, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chunguang YanHongwei Gao

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Citations

Mar 26, 2013·American Journal of Respiratory Cell and Molecular Biology·Verena AumillerMelanie Königshoff
Jul 6, 2015·Progress in Neurobiology·Marta Pulido-SalgadoJosep Saura
Sep 7, 2019·International Journal of Molecular Sciences·Rajwali KhanZan Linsen
Aug 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chunguang YanXimo Wang

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Methods Mentioned

BETA
bronchoalveolar lavage
PCR
ELISA
transfection
transmission electron microscopy
ELISAs
reverse transcription-PCR
transfections
Assay
protein assay

Software Mentioned

Image J

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