CCCTC-binding factor mediates effects of glucose on beta cell survival

Cell Proliferation
Shanli TsuiL Lu

Abstract

Pancreatic islet β-cell survival is paramount for regulation of insulin activity and for maintaining glucose homeostasis. Recently, Pax6 has been shown to be essential for many vital functions in β-cells, although many molecular mechanisms of its homeostasis in β-cells remain unclear. The present study investigates novel effects of glucose- and insulin-induced CCCTC-binding factor (CTCF) activity on Pax6 gene expression as well as for subsequent effects of insulin-activated signalling pathways, on β-cell proliferation. Pancreatic β-TC-1-6 cells were cultured in DMEM and stimulated with high concentrations of glucose (5-125 mm); cell viability was assessed by MTT assay. Effects of CTCF on Pax6 were evaluated in the high glucose-induced environment and CTCF/Erk-suppressed cells, by promoter reporter and western blotting analyses. Increases in glucose and insulin concentrations upregulated CTCF and consequently downregulated Pax6 in β-cell survival and proliferation. Knocking-down CTCF directly affected Pax6 transcription through CTCF binding and blocked the response to glucose. Altered Erk activity mediated effects of CTCF on controlling Pax6 expression, which partially regulated β-cell proliferation. CTCF functioned as a molecul...Continue Reading

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Jun 24, 2014·Life Sciences·Sivasangari BalakrishnanChidambaram Prahalathan
Oct 10, 2015·Molecular and Cellular Endocrinology·Simranjeet KaurUNKNOWN Hvidoere International Study Group
Jan 4, 2021·Biochemical Genetics·Ensieh ShahvazianMasoud Mirzaei
May 17, 2015·Biology Open·Ujué FresánM Lluisa Espinàs

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