CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells

OncoTargets and Therapy
Cong ZhouChun-Ting Wang

Abstract

Tumor growth is one of the most lethal attributes of human malignancy. The expression of CCDC7, a novel gene which has multiple functions, has been shown to be associated with tumor growth and poor prognosis in patients with cancer. However, the specific functions of CCDC7 remain unclear. Here, we investigated the molecular mechanisms underlying the effects of CCDC7 on proliferation in cervical cancer. The MTT and EdU assays were performed to evaluate the function of CCDC7. The immunohistochemical, quantitative real-time PCR (qRT-PCR), ELISA and Western blot assay were used to detect the gene and protein expression in tissues and cells. A xenograft test was conducted to detect the impact of CCDC7 on tumor development in vivo . In immunohistochemical analysis of 193 cases, normal cervical tissue and cervical cancer tissue show that CCDC7 expression is closely correlated with the development of cervical cancer and was positively correlated with the clinical stage and histological grade. Overexpression or knockdown of CCDC7 affected cell proliferation in cervical cancer cells in vitro. In a nude mouse xenograft model in vivo, knockdown of CCDC7 inhibited cell proliferation and tumor growth. Furthermore, CCDC7 overexpression upregu...Continue Reading

References

Mar 22, 2002·The Lancet Oncology·D M Parkin
Feb 18, 2004·Nature Reviews. Cancer·Hua Yu, Richard Jove
May 22, 2004·Journal of Biomedical Science·Shih-Pei HuangJaw-Town Lin
Nov 21, 2007·Acta Obstetricia Et Gynecologica Scandinavica·Shen YangmeiZhao Xia
Oct 24, 2009·Nature Reviews. Cancer·Hua YuRichard Jove
Mar 26, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Tasnim Ara, Yves A Declerck
May 19, 2010·Cell Division·Andrew JarnickiMatthias Ernst
Oct 26, 2011·Oncology Reports·Yang-Mei ShenXiao-Feng Sun
Sep 24, 2013·JAK-STAT·Eirini Bournazou, Jacqueline Bromberg
Oct 1, 2013·Pharmacology & Therapeutics·Xin YaoYihong Yao
Jan 29, 2014·JAK-STAT·Yong Tang, Xiuchun Cindy Tian
Feb 21, 2014·Seminars in Immunology·Koji Taniguchi, Michael Karin
Apr 8, 2014·The Oncologist·Javier MunozDavid S Hong
Apr 15, 2014·Cellular Signalling·Bing SunTeruyuki Nagamune
Feb 26, 2015·Journal of the National Cancer Institute·Altaf A DarMohammed Kashani-Sabet
Nov 13, 2015·International Journal of Cancer. Journal International Du Cancer·Kasturi Banerjee, Haluk Resat
Feb 2, 2019·Molecular Immunology·Alexander Vargas-Hernández, Lisa R Forbes

❮ Previous
Next ❯

Citations

May 5, 2021·Reproductive Sciences·Patra Priyadarshini Priyanka, Suresh Yenugu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Transfection
ELISA
FCS
electrophoresis
xenograft
xenografts
enzyme-linked immunosorbent assay
transfect
nuclear translocation

Software Mentioned

DPC
GraphPad Prism
Olympus

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.