CCL17/TARC and CCR4 expression in Merkel cell carcinoma

Oncotarget
Kashif RasheedUgo Moens

Abstract

Merkel cell carcinoma (MCC) is a rare, highly aggressive neuroendocrine skin cancer. In more than 80% of the cases, Merkel cell polyomavirus (MCPyV) is a causal factor. The oncogenic potential of MCPyV is mediated through its viral oncoproteins, large T antigen (LT) and small t antigen (sT). To investigate the role of cytokines in MCC, a PCR array analysis for genes encoding inflammatory cytokines and receptors was performed on MCPyV-negative and MCPyV-positive MCC cell lines, respectively. We detected an increased expression of CCL17/TARC in the MCPyV-positive MKL2 cell line compared to the MCPyV-negative MCC13 cell line. Transfection studies in MCC13 cells with LT expression plasmid, and a luciferase reporter plasmid containing the CCL17/TARC promoter, exhibited stimulated promoter activity. Interestingly, the ectopic expression of CCL17/TARC upregulated MCPyV early and late promoter activities in MCC13 cells. Furthermore, recombinant CCL17/TARC activated both the mitogen-activated protein kinase and the NF-κB pathways. Finally, immunohistochemical staining on human MCC tissues showed a strong staining of CCL17/TARC and its receptor CCR4 in both LT-positive and -negative MCC. Taken together, CCL17/TARC and CCR4 may be a poten...Continue Reading

References

Jul 30, 1995·International Journal of Radiation Oncology, Biology, Physics·J H LeonardG W Birrell
Sep 11, 2001·Biochemical and Biophysical Research Communications·Y FigenschauB Sveinbjörnsson
Aug 16, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·M H VermeerR Willemze
Sep 1, 2004·Experimental Dermatology·Christian VestergaardMette Deleuran
Oct 7, 2004·The Lancet Oncology·Michael Poulsen
Jan 20, 2005·The British Journal of Dermatology·C JohansenL Iversen
Jul 25, 2006·Journal of Dermatological Science·Hidehisa Saeki, Kunihiko Tamaki
Sep 6, 2006·Cancer Science·Takashi Ishida, Ryuzo Ueda
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kazuki HiraharaThomas S Kupper
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Traci L NessSteven L Kunkel
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martha M MonickGary W Hunninghake
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hyung W LimChang H Kim
Jan 15, 2008·Human Immunology·Elisabeth MaierDaniel Hebenstreit
Jan 19, 2008·Science·Huichen FengPatrick S Moore
Jan 29, 2008·International Journal of Cancer. Journal International Du Cancer·Yoshiki MizukamiHideki Fujii
Feb 16, 2008·British Journal of Haematology·Marijke NiensAnke van den Berg
Feb 19, 2008·Journal of the American Academy of Dermatology·Michelle HeathPaul Nghiem
Jun 19, 2008·Virology·Johanna R Abend, Michael J Imperiale
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Masahiro ShudaYuan Chang
Oct 22, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Terufumi KuboNoriyuki Sato
Nov 14, 2008·The Journal of Investigative Dermatology·Jules GiletAnne Tsicopoulos
Jun 6, 2009·International Journal of Cancer. Journal International Du Cancer·Yanis L TolstovPatrick S Moore
Dec 17, 2009·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·T MaruyamaH Fujii
Feb 24, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kazuhito YamamotoRyuzo Ueda
Mar 23, 2010·Cell·Sergei I GrivennikovMichael Karin
Apr 20, 2010·Advances in Cancer Research·Ole Gjoerup, Yuan Chang
Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Susanne StutteIrmgard Förster
Mar 18, 2011·International Journal of Cancer. Journal International Du Cancer·Roland HoubenDavid Schrama
Apr 12, 2011·Breast Cancer Research and Treatment·Ji-Yu LiZhi-Ming Shao
Jun 8, 2011·International Journal of Biological Sciences·Brian F Zamarron, WanJun Chen
Jul 30, 2011·PloS One·Huichen FengPatrick S Moore
Sep 29, 2011·Annual Review of Pathology·Yuan Chang, Patrick S Moore
Feb 9, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Takashi IshidaRyuzo Ueda
Apr 28, 2012·Current Hematologic Malignancy Reports·Kensei TobinaiShiro Akinaga
Jan 1, 2011·Frontiers in Immunology·Michelle L McCully, Bernhard Moser
Feb 27, 2013·The Journal of Investigative Dermatology·Sabrina AngermeyerRoland Houben

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Citations

Jan 30, 2019·Current Opinion in Oncology·Véronique Del Marmol, Celeste Lebbé
May 28, 2019·Frontiers in Cell and Developmental Biology·Oindrila BhattacharjeeSrikala Raghavan
Aug 15, 2019·International Journal of Molecular Sciences·Ugo Moens, Andrew Macdonald
Jul 9, 2020·Cancers·Valeria PietropaoloUgo Moens

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Methods Mentioned

BETA
profiler
PCR
transfection
Assay
protein

Software Mentioned

GraphPad
imageJ
Light Cycler 96 SW

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