CCL2 upregulation triggers hypoxic preconditioning-induced protection from stroke.

Journal of Neuroinflammation
Ann M StoweJeffrey M Gidday

Abstract

A brief exposure to systemic hypoxia (i.e., hypoxic preconditioning; HPC) prior to transient middle cerebral artery occlusion (tMCAo) reduces infarct volume, blood-brain barrier disruption, and leukocyte migration. CCL2 (MCP-1), typically regarded as a leukocyte-derived pro-inflammatory chemokine, can also be directly upregulated by hypoxia-induced transcription. We hypothesized that such a hypoxia-induced upregulation of CCL2 is required for HPC-induced ischemic tolerance. Adult male SW/ND4, CCL2-null, and wild-type mice were used in these studies. Cortical CCL2/CCR2 message, protein, and cell-type specific immunoreactivity were determined following HPC (4 h, 8% O2) or room air control (21% O2) from 6 h through 2 weeks following HPC. Circulating leukocyte subsets were determined by multi-parameter flow cytometry in naïve mice and 12 h after HPC. CCL2-null and wild-type mice were exposed to HPC 2 days prior to tMCAo, with immunoneutralization of CCL2 during HPC achieved by a monoclonal CCL2 antibody. Cortical CCL2 mRNA and protein expression peaked at 12 h after HPC (both p < 0.01), predominantly in cortical neurons, and returned to baseline by 2 days. A delayed cerebral endothelial CCL2 message expression (p < 0.05) occurred 2...Continue Reading

References

Jul 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J IvackoF S Silverstein
Sep 8, 2001·Stroke; a Journal of Cerebral Circulation·L WeiT A Woolsey
Mar 14, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Paula M HughesChristoph Wiessner
Mar 29, 2002·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Myriam BernaudinPascale Schumann-Bard
Apr 19, 2002·Journal of Neuroimmunology·Sulpicio G SorianoYang Pan
Oct 22, 2002·American Journal of Physiology. Heart and Circulatory Physiology·U MausJ Lohmeyer
Jun 28, 2003·Stroke; a Journal of Cerebral Circulation·Konstantin PrassAndreas Meisel
Jul 11, 2003·Annals of the New York Academy of Sciences·Anya M Y LinLow-Tone Ho
Oct 17, 2003·High Altitude Medicine & Biology·Keisho KatayamaMiharu Miyamura
Jul 6, 2004·Nature Medicine·Daniel J CeradiniGeoffrey C Gurtner
Mar 15, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Jeffrey M GiddayT S Park
Apr 15, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Tatsuro NishiPak H Chan
Sep 1, 2005·Molecular and Cellular Biochemistry·Cacchio MarisaMarcella Reale
May 23, 2006·Nature Reviews. Neuroscience·Jeffrey M Gidday
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria Carla BoscoLuigi Varesio
Aug 5, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Svetlana M StamatovicAnuska V Andjelkovic
Nov 23, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Erin E McCandlessRobyn S Klein
Dec 28, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yi-Ping YanRobert J Dempsey
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne R GockeAmy E Lovett-Racke
Feb 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John J SheehanStella E Tsirka
Mar 3, 2007·Stroke; a Journal of Cerebral Circulation·Oliver B DimitrijevicAnuska V Andjelkovic
May 19, 2007·Journal of Neuroscience Research·Xian Shuang LiuMichael Chopp
Feb 2, 2008·Stroke; a Journal of Cerebral Circulation·Satoshi TeraoD Neil Granger
Feb 22, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Grace VangeisonDavid A Rempe
Mar 28, 2008·The Journal of Biological Chemistry·Jianli NiuPappachan E Kolattukudy
Jun 26, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adám DénesKrisztina J Kovács
Apr 10, 2009·Investigative Ophthalmology & Visual Science·Athanasios DagkalisIsabel J Crane
Apr 28, 2009·Neurobiology of Disease·Ann M StoweJeffrey M Gidday
May 19, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Satoshi TaieMasaaki Ueki
May 26, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Danielle L BurkeKurt R Stenmark

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Citations

Sep 21, 2013·Cellular and Molecular Life Sciences : CMLS·Yao Yao, Stella E Tsirka
Dec 11, 2013·Mediators of Inflammation·Pawel Wolinski, Andrzej Glabinski
Mar 14, 2014·Frontiers in Neuroscience·Lidia Garcia-BonillaJosef Anrather
Jun 13, 2014·Frontiers in Cellular Neuroscience·Jessica L WilliamsRobyn S Klein
Sep 17, 2016·Journal of Immunology Research·Constantino Tomas-SanchezBertha Alicia Leon-Chavez
Sep 13, 2016·Frontiers in Neurology·Wentao LiFernando D Testai
Dec 6, 2016·International Journal of Molecular Sciences·Meredith Harrison-BrownRichard Banati
May 6, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Courtney Lane-DonovanAnn M Stowe
Jun 21, 2017·Neurochemistry International·Chen ChenNai-Hong Chen
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Adel HelmyPeter J Hutchinson
Mar 24, 2016·International Journal of Molecular Sciences·Zhen JinLiang-Jun Yan
Nov 1, 2018·American Journal of Physiology. Cell Physiology·Changjun YangEduardo Candelario-Jalil
Jun 9, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Roger A FecherGeorge S Deepe
Oct 21, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Uma Maheswari SelvarajAnn M Stowe
Jun 2, 2017·Experimental and Therapeutic Medicine·Liming QingYihe Hu
Aug 24, 2019·Frontiers in Neuroscience·Minna Yli-KarjanmaaKate Lykke Lambertsen
Jul 25, 2019·International Journal of Molecular Sciences·Christine SilwedelKirsten Glaser
Apr 19, 2017·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Seyed Esmaeil KhoshnamHadi Fathi Moghaddam
Jul 10, 2019·Frontiers in Pharmacology·Kyle MaloneChristian Waeber
Oct 25, 2020·International Journal of Molecular Sciences·Sanghun LeeNam Keun Kim

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

Prism
Flowjo
GraphPad
Diva

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