CCL25-CCR9 interaction modulates ovarian cancer cell migration, metalloproteinase expression, and invasion.

World Journal of Surgical Oncology
Erica JohnsonJames W Lillard

Abstract

Ovarian carcinoma (OvCa) is the most lethal gynecological malignancy among women and its poor prognosis is mainly due to metastasis. Chemokine receptor CCR9 is primarily expressed by a small subset of immune cells and its only natural ligand, CCL25, is largely expressed in the thymus, which involutes with age. Other than the thymus, CCL25 is expressed by the small bowel. Interactions between CCL25 and CCR9 have been implicated in leukocyte trafficking to the small bowel, a frequent metastatic site for OvCa cells. The current study shows OvCa tissue and cells significantly express CCR9, which interacts with CCL25 to support carcinoma cell migration and invasion. RT-PCR and flow cytometry techniques were used to quantify the expression CCR9 by OvCa cells. OvCa tissue microarrays (TMA) was used to confirm CCR9 expression in clinical samples. The Aperio ScanScope scanning system was used to quantify immunohistochemical staining. Cell invasion and migration assays were performed using cell migration and matrigel invasion chambers. Matrix metalloproteinase (MMP) mRNAs were quantified by RT-PCR and active MMPs were quantified by ELISA. Our results show significantly (p<0.001) higher expression of CCR9 by mucinous adenocarcinoma, papil...Continue Reading

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Datasets Mentioned

BETA
X00686.1

Methods Mentioned

BETA
fluorescence-activated cell-sorting
flow cytometry
xenografts

Software Mentioned

Primer
SAS
ImageScope
Stat view
Cell Quest
CellQuest
Aperio ImageScope
ScanScope

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