CcpA and three newly identified proteins are involved in biofilm development in Lactobacillus plantarum

Journal of Basic Microbiology
Lidia MuscarielloMargherita Sacco

Abstract

The aim of this study was to identify genes involved in biofilm development in the probiotic lactic acid bacterium Lactobacillus plantarum. The ability of L. plantarum LM3 and of some derivative mutant strains to form biofilm has been investigated. Biofilm microtitre plate assays showed that L. plantarum LM3-2, carrying a null mutation in the ccpA gene, coding the CcpA master regulator, was partially impaired in biofilm production compared to wild type (LM3). Moreover, we found three genes in the L. plantarum genome, hereby named flmA, flmB, and flmC, whose deduced amino acid sequences show significant identity with the Streptococcus mutans BrpA (biofilm regulatory protein A). We investigated the role of FlmA, FlmB, and FlmC in biofilm formation by isolating strains carrying null mutations in the corresponding genes. Our results suggest involvement of the Flm proteins in biofilm development. Moreover, transcriptional studies show that expression of flmA, flmB, and flmC is under the control of CcpA. These results, together with the reduced ability of LM3-2 (ccpA1) to form biofilm, strongly suggest a positive role of the master regulator CcpA in biofilm development.

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Citations

Sep 5, 2014·Microbial Cell Factories·François P Douillard, Willem M de Vos
Sep 27, 2014·International Journal of Oral Science·Claudia VuottoGianfranco Donelli
Nov 8, 2015·Archives of Microbiology·Valeria VastanoLidia Muscariello
Apr 29, 2014·Journal of Basic Microbiology·Eisha MhatreAkos T Kovács
May 24, 2015·International Journal of Food Microbiology·Maria João Fraqueza
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Jan 23, 2021·International Journal of Molecular Sciences·Cordula StefanovićChristina Schäffer
Nov 20, 2021·Environmental Science and Pollution Research International·Anuja MaitreyaHemant J Purohit

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