CCR2 Contributes to F4/80+ Cells Migration Along Intramembranous Bone Healing in Maxilla, but Its Deficiency Does Not Critically Affect the Healing Outcome

Frontiers in Immunology
Claudia Cristina BiguettiGustavo Pompermaier Garlet

Abstract

Bone healing depends of a transient inflammatory response, involving selective migration of leukocytes under the control of chemokine system. CCR2 has been regarded as an essential receptor for macrophage recruitment to inflammation and healing sites, but its role in the intramembranous bone healing on craniofacial region remains unknown. Therefore, we investigated the role of CCR2 on F4/80+ cells migration and its consequences to the intramembranous healing outcome. C57BL/6 wild-type (WT) and CCR2KO mice were subjected to upper right incisor extraction, followed by micro-computed tomography, histological, immunological, and molecular analysis along experimental periods. CCR2 was associated with F4/80+ cells influx to the intramembranous bone healing in WT mice, and CCR2+ cells presented a kinetics similar to F4/80+ and CCR5+ cells. By contrast, F4/80+ and CCR5+ cells were significantly reduced in CCR2KO mice. The absence of CCR2 did not cause major microscopic changes in healing parameters, while molecular analysis demonstrated differential genes expression of several molecules between CCR2KO and WT mice. The mRNA expression of TGFB1, RUNX2, and mesenchymal stem cells markers (CXCL12, CD106, OCT4, NANOG, and CD146) was decreas...Continue Reading

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Citations

May 13, 2020·Journal of Periodontology·Franz Josef StraussReinhard Gruber
Apr 27, 2019·Frontiers in Immunology·Claudia Cristina BiguettiGustavo Pompermaier Garlet
Aug 28, 2020·Frontiers in Endocrinology·Claudia Cristina BiguettiMariza Akemi Matsumoto
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Yasuhito YaharaBenjamin A Alman

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Methods Mentioned

BETA
PCR
Flow Cytometric
flow cytometry
FACS

Software Mentioned

NRecon
Adobe Photoshop
CellQuest
CT
CTAn
Vox
CTVox
GraphPad Prism
GraphPad
FACScan

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