CCR2 deficiency results in increased osteolysis in experimental periapical lesions in mice

Journal of Endodontics
Thiago Pompermaier GarletFernando de Queiroz Cunha

Abstract

Periapical lesions are chronic inflammatory disorders of periradicular tissues caused by etiologic agents of endodontic origin. The inflammatory chemokines are thought to be involved in the latter observed osteolysis. With a murine model of experimental periapical lesion, the objective of this study was to evaluate the role of the chemokine receptor CCR2 in the lesion progression, osteoclast differentiation and activation, and expression of inflammatory osteolysis-related mediators. For lesion induction, right mandibular first molars were opened surgically with a 1/4 carbine bur, and 4 bacterial strains were inoculated in the exposed dental pulp; left mandibular first molars were used as controls. Animals were killed at 3, 7, 14, and 21 days after surgeries to evaluate the kinetics of lesion development. CCR2 KO mice showed wider lesions than WT mice. CCR2 KO mice also expressed higher levels of the osteoclastogenic and osteolytic factors, receptor activator of nuclear factor kappa B ligand (RANKL) and cathepsin K, of the proinflammatory cytokine tumor necrosis factor-alpha, and of the neutrophil migration related chemokine, KC. These results suggest that CCR2 is important in host protection to periapical osteolysis.

Citations

May 7, 2011·Journal of Oral Microbiology·Dana T GravesGustavo P Garlet
Feb 26, 2011·Clinical & Developmental Immunology·Celso Martins Queiroz-JuniorTarcília Aparecida da Silva
Nov 10, 2015·Ultrastructural Pathology·Katharina Morant Holanda de OliveiraRaquel Assed Bezerra da Silva
Apr 29, 2014·Journal of Endodontics·Chunyan WanZhi Chen
Dec 12, 2012·Journal of Endodontics·Andreza Maria Fabio AranhaGustavo Pompermaier Garlet
May 19, 2012·Journal of Endodontics·Raquel Assed Bezerra da SilvaLea Assed Bezerra Silva
Jan 31, 2012·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Silvana Rodrigues de Albuquerque TaddeiTarcília Aparecida da Silva
Aug 21, 2012·International Endodontic Journal·G N BelibasakisM Zehnder
Nov 13, 2012·Journal of Dental Research·T TsutsumiK Okabe
Jan 26, 2021·International Endodontic Journal·V NagendrababuP M H Dummer

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