CCR3 Monoclonal Antibody Inhibits Eosinophilic Inflammation and Mucosal Injury in a Mouse Model of Eosinophilic Gastroenteritis

Allergy, Asthma & Immunology Research
Dae Jin SongJi Tae Choung

Abstract

Although the role of eosinophils in eosinophilic gastroenteritis (EGE) is not fully understood, they are believed to be a principal effector cell. Previous studies have demonstrated that eotaxin and its specific receptor, cysteine-cysteine chemokine receptor-3 (CCR3), play a central role in eosinophil trafficking into the gastrointestinal (GI) tract. Thus, we examined the targeting of CCR3 as a potential therapeutic intervention for EGE in a mouse model. Eight- to 10-week-old BALB/c mice were intraperitoneally sensitized and intragastrically challenged with ovalbumin (OVA). Different groups of mice were administered either an anti-CCR3 antibody or a control IgG by intraperitoneal injection 1 hour before each OVA challenge. Eosinophilic inflammation in the intestinal mucosa, mucosal injury, and severity of diarrhea were compared between different groups at 1 hour after final OVA challenge. Anti-CCR3 antibody reduced the number of eosinophils in peripheral blood and intestinal mucosa, but not in bone marrow. This reduction was associated with restoration of reduced villous crypt ratio, increased intestinal epithelial cell proliferation, and weight loss induced by OVA challenge. However, Anti-CCR3 antibody had no effect on the lev...Continue Reading

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Citations

Jul 4, 2018·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R T FilipponeK Nurgali
Mar 12, 2019·Inflammatory Bowel Diseases·Rhiannon T FilipponeKulmira Nurgali
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Oct 24, 2019·Current Pediatric Reviews·Amelia LicariGian Luigi Marseglia

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
protein assay
ELISA
antisense oligonucleotides

Software Mentioned

Image
Graph Pad Prism
Pro Plus

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