Ccr4-Not and TFIIS Function Cooperatively To Rescue Arrested RNA Polymerase II

Molecular and Cellular Biology
Arnob DuttaJoseph C Reese

Abstract

Expression of the genome requires RNA polymerase II (RNAPII) to transcribe across many natural and unnatural barriers, and this transcription across barriers is facilitated by protein complexes called elongation factors (EFs). Genetic studies in Saccharomyces cerevisiae yeast suggest that multiple EFs collaborate to assist RNAPII in completing the transcription of genes, but the molecular mechanisms of how they cooperate to promote elongation are not well understood. The Ccr4-Not complex participates in multiple steps of mRNA metabolism and has recently been shown to be an EF. Here we describe how Ccr4-Not and TFIIS cooperate to stimulate elongation. We find that Ccr4-Not and TFIIS mutations show synthetically enhanced phenotypes, and biochemical analyses indicate that Ccr4-Not and TFIIS work synergistically to reactivate arrested RNAPII. Ccr4-Not increases the recruitment of TFIIS into elongation complexes and enhances the cleavage of the displaced transcript in backtracked RNAPII. This is mediated by an interaction between Ccr4-Not and the N terminus of TFIIS. In addition to revealing insights into how these two elongation factors cooperate to promote RNAPII elongation, our study extends the growing body of evidence suggestin...Continue Reading

References

Aug 16, 1994·Proceedings of the National Academy of Sciences of the United States of America·G Cipres-Palacin, C M Kane
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·C JeonK Agarwal
Dec 20, 1999·The Journal of Biological Chemistry·Q YanR C Conaway
Mar 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Wind, D Reines
Sep 6, 2002·Biochimica Et Biophysica Acta·Rachel N Fish, Caroline M Kane
Jul 29, 2003·Progress in Nucleic Acid Research and Molecular Biology·Clyde L Denis, Junji Chen
Oct 11, 2003·Trends in Genetics : TIG·Karen M Arndt, Caroline M Kane
Jan 10, 2004·Methods in Enzymology·Joseph C Reese, Michael R Green
Oct 19, 2004·Genes & Development·Robert J SimsDanny Reinberg
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Murali PalangatRobert Landick
Nov 23, 2005·Proceedings of the National Academy of Sciences of the United States of America·Man-Hee SuhJianhua Fu
May 2, 2006·Genetics·Rachel N FishCaroline M Kane
Mar 14, 2007·Trends in Biochemical Sciences·Jesper Q Svejstrup
Oct 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Bong KimJeffrey A Ranish
Jun 25, 2008·Annual Review of Biophysics·P CramerA Vannini
Jul 17, 2008·Genes & Development·Yad Ghavi-HelmJulie Soutourina
Jun 6, 2009·Annual Review of Biochemistry·Evgeny Nudler
Oct 14, 2009·Molecular and Cellular Biology·Daniel S GinsburgAlan G Hinnebusch
Sep 8, 2010·Nature Structural & Molecular Biology·Andreas MayerPatrick Cramer
Feb 25, 2011·Nature·Alan C M Cheung, Patrick Cramer
Mar 17, 2011·Genes & Development·Jennifer A KrukJoseph C Reese
Oct 27, 2011·Gene·Martine A Collart, Olesya O Panasenko
Mar 16, 2012·Critical Reviews in Biochemistry and Molecular Biology·Jason E Miller, Joseph C Reese
Jul 24, 2012·FEBS Letters·Fernando Gómez-HerrerosSebastián Chávez
Sep 15, 2012·Biochimica Et Biophysica Acta·Joseph C Reese
Sep 18, 2012·Biochimica Et Biophysica Acta·Fuensanta W Martinez-Rucobo, Patrick Cramer
Sep 28, 2013·Science·Kenji MurakamiRoger D Kornberg

❮ Previous
Next ❯

Citations

Jul 22, 2015·Biomolecules·Leonardo C Andrade-LimaMats Ljungman
Jan 30, 2016·Wiley Interdisciplinary Reviews. RNA·Martine A Collart
Apr 29, 2016·Molecular Biology of the Cell·Alfonso Rodriguez-GilM Lienhard Schmitz
Jul 21, 2016·Nature Reviews. Molecular Cell Biology·Tatiana García-Muse, Andrés Aguilera
Apr 6, 2017·Nucleic Acids Research·John B CrickardJoseph C Reese
Apr 14, 2017·Genome Research·Cornelia BrönnerMario Halic
Jul 14, 2017·Microbiology and Molecular Biology Reviews : MMBR·Yeva Mirzakhanyan, Paul D Gershon
May 23, 2019·Molecular Plant Pathology·Hugo Massayoshi ShimoCelso Eduardo Benedetti
Jan 4, 2017·Molecular Systems Biology·Kevin M Harlen, L Stirling Churchman
Aug 9, 2019·Nucleic Acids Research·Victoria BegleySebastián Chávez
Nov 29, 2015·Biochemical Society Transactions·Zoltan Villanyi, Martine A Collart
Aug 14, 2020·Wiley Interdisciplinary Reviews. RNA·Yong-Bin Yan
Jun 11, 2020·The Journal of Biological Chemistry·Jonathan FischerMordechai Choder
Sep 29, 2019·Nature Reviews. Molecular Cell Biology·Hannes LansJurgen A Marteijn
Jun 16, 2017·Scientific Reports·Alfonso Rodríguez-GilM Lienhard Schmitz
May 21, 2019·Frontiers in Plant Science·Wesam M Al KhateebDana F Schroeder
Nov 4, 2020·Cells·Nafiseh Chalabi Hagkarim, Roger J Grand
Nov 20, 2020·Nature Reviews. Molecular Cell Biology·Melvin Noe GonzalezJesper Q Svejstrup
Dec 6, 2020·Genes·Wojciech StrzałkaAgnieszka Katarzyna Banaś
Aug 23, 2021·DNA Repair·Oluwasegun T Akinniyi, Joseph C Reese

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved