CCR5 ligands modulate CXCL12-induced chemotaxis, adhesion, and Akt phosphorylation of human cord blood CD34+ cells.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Sunanda Basu, Hal E Broxmeyer

Abstract

CXCL12 and its receptor CXCR4 play an important role in hematopoietic stem/progenitor cell (HSPC) migration from and retention within the bone marrow. HSPCs are very selective in their chemotactic response and undergo chemotaxis only in response to CXCL12. In addition to CXCR4, HSPCs express receptors for various other chemokines; however, the role of these receptors is not well understood. Freshly isolated CD34(+) cells (highly enriched for HSPCs) from cord blood (CB) express low levels of CCR5; however, if the cells were washed with acidic buffer before Ab staining to remove any ligand bound to CCR5, then nearly 80% of CD34(+) CB cells were found to express CCR5 on the cell surface. Although none of the CCR5 ligands investigated in this study (CCL3, CCL4, and CCL5) induced chemotaxis, at relatively high concentrations they transiently enhanced CXCL12-mediated chemotaxis of CD34(+) CB cells. In contrast, CXCL12-mediated adhesion of cells to VCAM-1-coated surfaces was reduced if CD34(+) CB cells were pretreated with these CCR5 ligands for 15 min. The effect of these chemokines on CXCL12-mediated responses was not at the level of CXCR4 expression, but on downstream signaling pathways elicited by CXCL12. Pretreatment with CCR5 ch...Continue Reading

References

Jan 1, 1988·Critical Reviews in Oncology/hematology·H E Broxmeyer, D E Williams
Apr 1, 1984·The Journal of Cell Biology·T H Howard, W H Meyer
Nov 7, 1993·Biochimica Et Biophysica Acta·M J Lohse
Nov 1, 1996·Trends in Pharmacological Sciences·T T ChuangA De Blasi
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·M BhatiaJ E Dick
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q MaT A Springer
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·H H ChuangL Y Jan
Feb 26, 2000·Immunology Today·J D SedgwickH Körner
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·C Y ChungR A Firtel
Sep 5, 2001·Journal of Virology·F BaribaudR W Doms
May 8, 2002·The Journal of Experimental Medicine·Douglas E WrightIrving L Weissman
Jun 14, 2002·Cell·Miho Iijima, Peter Devreotes
Oct 24, 2002·Blood·Joachim RolandMartine Biard-Piechaczyk
Nov 16, 2002·The Journal of Biological Chemistry·Gregory J BabcockJoseph Sodroski
Dec 25, 2002·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Katrien PrincenDominique Schols
Feb 22, 2003·Blood·Thalia PapayannopoulouBetty Nakamoto
Aug 2, 2003·Journal of Cell Science·Sylvain Merlot, Richard A Firtel
Oct 2, 2003·Current Opinion in Cell Biology·Christopher V Carman, Timothy A Springer
Jul 1, 2004·The Journal of Biological Chemistry·Gordon J ZwartzLarry A Sklar
Apr 20, 2005·The Journal of Experimental Medicine·Hal E BroxmeyerEdward F Srour
Oct 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Asit K DeRonald G Tompkins
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandre ChigaevLarry A Sklar
Aug 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sunanda BasuHal E Broxmeyer
Jan 12, 2008·Journal of Cell Science·Bryan HeitPaul Kubes
Jul 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rita Lucia ContentoAntonella Viola

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Citations

Oct 30, 2013·The Journal of Experimental Medicine·Tomohisa BabaNaofumi Mukaida
Feb 23, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·J Davin MillerLawrence S Prince
Jun 27, 2014·Frontiers in Physiology·Yvonne DöringHeidi Noels
Oct 30, 2010·Circulation Research·Sebastian F Mause, Christian Weber
Jan 8, 2017·The Journal of Pharmacology and Experimental Therapeutics·William G RobichauxJoe B Blumer
Oct 12, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katarzyna FranciszkiewiczFathia Mami-Chouaib
Mar 1, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Oliver GiegoldHeinfried H Radeke
Jul 3, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yedidya SaimanMeena B Bansal
Feb 2, 2021·Endocrine Reviews·Adil Rasheed, Katey J Rayner

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