CD107a as a marker of activation in chicken cytotoxic T cells

Journal of Immunological Methods
Eva WattrangHelle R Juul-Madsen

Abstract

The study aimed to evaluate cell surface mobilisation of CD107a as a general activation marker on chicken cytotoxic T cells (CTL). Experiments comprised establishment of an in vitro model for activation-induced CD107a mobilisation and design of a marker panel for the detection of CD107a mobilisation on chicken CTL isolated from different tissues. Moreover, CD107a mobilisation was analysed on CTL isolated from airways of infectious bronchitis virus (IBV)-infected birds direct ex vivo and upon in vitro stimulation. Results showed that phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin was a consistent inducer of CD107a cell surface mobilisation on chicken CTL in a 4h cell culture model. In chickens experimentally infected with IBV, higher frequencies of CTL isolated from respiratory tissues were positive for CD107a on the cell surface compared to those from uninfected control chickens indicating in vivo activation. Moreover, upon in vitro PMA+ ionomycin stimulation, higher proportions of CTL isolated from the airways of IBV-infected chickens showed CD107a mobilisation compared to those from uninfected control chickens. Monitoring of CD107a cell surface mobilisation may thus be a useful tool for studies of chicken...Continue Reading

References

May 1, 1986·The Journal of Cell Biology·J Lippincott-Schwartz, D M Fambrough
Mar 16, 2000·Developmental and Comparative Immunology·E W CollissonS H Seo
Sep 13, 2003·Veterinary Immunology and Immunopathology·Yongqiang WangOlli Vainio
Oct 7, 2003·Nature Medicine·Valerie RubioPeter P Lee
Oct 29, 2003·Journal of Immunological Methods·Michael R BettsRichard A Koup
Dec 18, 2004·Journal of Immunological Methods·Galit AlterMarcus Altfeld
Dec 14, 2007·Veterinary Immunology and Immunopathology·Guangliang LiuDonglai Wu
Oct 7, 2008·Cellular Immunology·Esin AktasGunnur Deniz
Mar 2, 2010·Developmental and Comparative Immunology·Christine A JansenLonneke Vervelde
Jun 4, 2010·Expert Review of Vaccines·Liubov ZaritskayaAnatoli M Malyguine
Jul 28, 2012·Veterinary Immunology and Immunopathology·Guangliang LiuDonglai Wu
Dec 19, 2012·Veterinary Immunology and Immunopathology·L VerveldeC A Jansen
Feb 26, 2013·European Journal of Immunology·Gang ZhouKevin P Kane
Jan 21, 2014·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Alfred BereJo-Ann S Passmore

❮ Previous
Next ❯

Citations

Feb 23, 2020·Cellular and Molecular Life Sciences : CMLS·Yi YangShaobin Shang
Feb 23, 2020·Human Immunology·A JacquierJ LeMaoult

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.