CD11c+ B Cells Are Mainly Memory Cells, Precursors of Antibody Secreting Cells in Healthy Donors.

Frontiers in Immunology
Marie-Laure GolinskiSébastien Calbo

Abstract

CD11c+ B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c+ B cells from healthy donors by flow cytometry, microarray analysis, and in vitro functional assays. Here, we report that CD11c+ B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if their phenotype is heterogeneous, with overexpression of genes involved in B-cell activation and differentiation as well as in antigen presentation. Upon activation, CD11c+ B cells can differentiate into antibody-secreting cells, and CD11c could be upregulated in CD11c- B cells by B-cell receptor activation. Finally, we show that patients with pemphigus, an autoimmune disease mediated by B cells, have a decreased frequency of CD11c+ B cell after treatment, relative to baseline. Our findings show that CD11c+ B cells are mainly memory B cells prone to differentiate into antibody secreting cells that accumulate with age, independently of gender.

References

Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J D LoikeS C Silverstein
Jan 1, 1990·Current Topics in Microbiology and Immunology·H Rosen, S K Law
Apr 1, 1995·The Journal of Experimental Medicine·R R Ingalls, D T Golenbock
May 23, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·R GarnotelP Gillery
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Stanford L PengLaurie H Glimcher
May 27, 2003·Nature Immunology·Ningshu LiuKevin B Bacon
Apr 16, 2004·Immunity·Michael J TownsendLaurie H Glimcher
Aug 6, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Takayuki YoshimotoJunichiro Mizuguchi
Sep 15, 2005·The Journal of Experimental Medicine·Götz R A EhrhardtMax D Cooper
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria C JenmalmJonathon D Sedgwick
Jul 31, 2007·Clinics in Laboratory Medicine·James W TungLeonore A Herzenberg
Apr 18, 2008·Current Opinion in Immunology·Frances E Lund
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 14, 2009·The Journal of Experimental Medicine·Fernando Cruz-GuillotyAnjana Rao
Jul 14, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Greta E WeissSusan K Pierce
Feb 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chunyang LienPaula M Pitha
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·David A SavitskyKenya Honda
Aug 29, 2012·Journal of Leukocyte Biology·Vera M KampLeo Koenderman
Sep 5, 2012·Immunologic Research·Anatoly V RubtsovPhilippa Marrack
Aug 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kira RubtsovaPhilippa Marrack
Aug 1, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wenyu LinSébastien Calbo
Jun 3, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anatoly V RubtsovPhilippa Marrack
Jun 5, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Judith LeitnerPeter Steinberger
Apr 15, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Huifang LiMate Tolnay
Jul 20, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Burton E BarnettE John Wherry
Nov 12, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nele ClaesVeerle Somers
Feb 28, 2017·The Journal of Clinical Investigation·Kira RubtsovaPhilippa Marrack
Jun 26, 2017·The Journal of Investigative Dermatology·Huijie YuanMeng Pan
Jan 13, 2018·Frontiers in Immunology·Leonie SchittenhelmVicky L Morrison
Feb 28, 2018·Nature Immunology·Michela ManniAlessandra B Pernis

❮ Previous
Next ❯

Citations

Nov 17, 2020·Frontiers in Immunology·Marlene E ReinckeBaerbel Keller
Mar 30, 2021·Frontiers in Immunology·Hector Rincon-ArevaloThomas Dörner
Nov 7, 2020·International Reviews of Immunology·Vijay Kumar
Feb 27, 2021·The Journal of Clinical Investigation·Rose-Marie RébillardCatherine Larochelle
May 11, 2021·Clinical and Experimental Immunology·Yuzi LiFanlei Hu
Jun 26, 2021·Frontiers in Immunology·Rick WilbrinkGwenny M Verstappen
Jun 26, 2021·Frontiers in Immunology·Wenhan DuLiwei Lu
Aug 23, 2021·Immunity & Ageing : I & a·Daniela FrascaSavita Pahwa

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE112515

Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
ELISA
PCR
Feature Extraction
chip
FACS
fluorescence-activated cell sorting
flow

Clinical Trials Mentioned

NCT00784589

Software Mentioned

GeneSpring
Time PCR Analysis
TreeStar
GenMAPP
Real
FlowJo
GeneSpring Single Experiment Analysis
Biomark HD
DAVID Bioinformatics Resources
Wikipathways

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

B cell Activation

B cell activation is initiated by the ligation of the B cell receptor with antigen and ultimately results in the production of protective antibodies against potentially pathogenic invaders. Here is the latest research.