CD14 and CD11b mediate serum-independent binding to human monocytes of an acylpolygalactoside isolated from Klebsiella pneumoniae.

Infection and Immunity
Z HmamaJ P Revillard

Abstract

A water-soluble acylpolygalactosyl (APG) of 34 kDa was obtained from the Klebsiella pneumoniae membrane by alkaline hydrolysis and delipidation. APG comprises a poly(1,3)galactose chain, a core, and a lipid moiety made of a glucosamine disaccharide with two N-linked beta OH-myristates. The monocyte binding sites for APG were investigated by flow cytometry. Biotin-labelled APG (Biot-APG) bound to monocytes at 4 degrees C in the absence of serum, calcium, and magnesium. The binding was dose dependent, saturable, and displaced by unlabelled APG. Neither the polysaccharide chain present in APG-related molecules nor the PPi group or additional ester-linked myristates and palmitates were required for APG binding. The role of CD11b and CD14 was demonstrated by competitive inhibition with monoclonal antibodies and by the uptake of APG by these solubilized proteins. APG was rapidly internalized into monocytes at 37 degrees C while CD14 and CD11b/CD18 molecules were partially down-modulated. Lipopolysaccharides (LPS) from the same K. pneumoniae strain and from Escherichia coli and Salmonella minnesota partially competed for Biot-APG binding in the absence but not in the presence of serum. When altered by alkaline hydrolysis, those LPS be...Continue Reading

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Citations

Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kristin H LongSimon L Newman
Jul 15, 2003·Clinical Microbiology Reviews·Edwin S Van AmersfoortJohan Kuiper
Jan 29, 2000·European Journal of Clinical Investigation·P Antal-Szalmás
Feb 28, 2003·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·J EspinozaB H Yoon
Mar 6, 1999·Current Opinion in Microbiology·D HeumannT Calandra
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Didier Heumann, Thierry Roger
May 1, 1996·Infection and Immunity·R LandmannW Zimmerli
Aug 31, 2021·JAMA Network Open·Christina A FlemingHenry P Redmond

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