CD14 and tissue factor expression by bacterial lipopolysaccharide-stimulated bovine alveolar macrophages in vitro.

Infection and Immunity
Z YangP N Bochsler

Abstract

The membrane-associated CD14 receptor (mCD14) is a monocyte/macrophage differentiation antigen, and it has been demonstrated to serve as a receptor for bacterial lipopolysaccharide (LPS; endotoxin). Binding of LPS to mCD14 has been shown to be associated with LPS-induced macrophage, monocyte, and neutrophil activation in humans. In this report, we describe the presence and function of an mCD14-like receptor on bovine alveolar macrophages (bAM). An immunofluorescence technique and flow cytometric analysis indicated binding of anti-human CD14 monoclonal antibodies (MAb) My4, 3C10, and 60bd to bAM. Binding of anti-CD14 MAb (3C10 and MY4) was reduced over 20% by pretreatment of bAM with phosphatidylinositol-specific phospholipase C (0.5 to 1.0 U/ml), indicating that bovine mCD14 is a glycosyl phosphatidylinositol-anchored protein. In addition, pretreatment of bAM with anti-CD14 MAb decreased binding of 125I-labeled LPS to macrophages, suggesting that bovine mCD14 serves as a receptor for LPS. A cDNA probe based on the human sequence for CD14 was used in Northern (RNA) blot analysis, and hybridization to human monocyte CD14 yielded the expected 1.5-kb band. Hybridization to bovine mRNA yielded a 1.5-kb band plus an unexpected 3.1-kb...Continue Reading

References

Dec 1, 1992·The Journal of Experimental Medicine·E A FreyS D Wright
Jan 1, 1992·Journal of Veterinary Internal Medicine·L O WhiteleyM S Kannan
Dec 31, 1991·Biochemical and Biophysical Research Communications·Y TakayenokiS Iwanaga
Jan 1, 1991·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·J MathisonR Ulevitch
Sep 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C R RaetzC F Nathan
Sep 21, 1990·Science·R R SchumannR J Ulevitch
Sep 1, 1986·The Journal of Experimental Medicine·P S TobiasR J Ulevitch
Jan 1, 1987·Methods in Enzymology·R J MacDonaldJ M Chirgwin
Jan 1, 1988·CRC Critical Reviews in Biochemistry·R R Bach
Dec 1, 1986·European Journal of Immunology·V BazilI Hilgert
Nov 1, 1986·Veterinary Pathology·J C LayW L Castleman
Jan 1, 1994·The Journal of Experimental Medicine·E HailmanS D Wright
Feb 1, 1993·Infection and Immunity·P GallayD Heumann
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E FerreroS M Goyert
Jan 1, 1993·Advances in Immunology·R J Ulevitch
Jan 1, 1994·Journal of Leukocyte Biology·H W Ziegler-HeitbrockJ G Haas
Jan 1, 1993·Advances in Pharmacology·P A Ward, M S Mulligan

❮ Previous
Next ❯

Citations

Dec 28, 2007·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·Thomas W Stief
Jul 19, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Lilian J Oliveira, P J Hansen
Aug 1, 1997·The Journal of Veterinary Medical Science·A IkedaK Sekikawa
May 19, 2006·Clinical and Applied Thrombosis/hemostasis : Official Journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis·T W Stief
Mar 10, 1999·The American Journal of Physiology·C Stamme, J R Wright
Feb 14, 2018·Journal of Thrombosis and Haemostasis : JTH·E W SkjefloT E Mollnes
Sep 1, 1996·Veterinary Immunology and Immunopathology·G L MasonP N Bochsler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.