PMID: 7543706Aug 1, 1995Paper

CD14-lipopolysaccharide receptor activity in hepatic macrophages after cholestatic liver injury

Surgery
T F Tracy, E S Fox

Abstract

Increased septic complications and altered cytokine responses to bacterial endotoxins are lethal consequences of chronic liver disease. Kupffer cells (HM phi) control the clearance of lipopolysaccharide (LPS) and subsequent cytokine responses. The purpose of this study is to determine the phenotype and function of HM phi after cholestatic liver injury. Male Sprague-Dawley rats underwent 4 days of common bile duct division and ligation or sham laparotomy. HM phi were isolated by collagenase-pronase perfusion and purified by centrifugal elutriation. Indirect immunofluorescence and Western blot analysis with monoclonal antibodies OX42 and ED9 showed that HM phi from cholestatic rats expressed MAC-1 (CD11b) and the LPS receptor CD14, respectively. HM phi from sham animals were negative for CD14 and CD11b by use of immunofluorescence; however, Western blot detected low levels of CD14 in controls. Functional analysis for tumor necrosis factor-alpha release after LPS stimulation showed that HM phi isolated from cholestatic livers exhibited serum (normal rat serum) dependent stimulation (1 ng/ml LPS + 1% normal rat serum; n = 12; p < 0.05 at 8 hours, t test) consistent with the presence of CD14 on their cell surface. This study shows t...Continue Reading

Citations

May 15, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H W HarrisP Chau
Mar 6, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·J Bauer
Jun 26, 2002·Journal of the American College of Surgeons·Farnaz N MoazzamJin Wen Ding
Jul 2, 1998·Clinical and Experimental Immunology·G RoglerV Gross
Jan 31, 2009·Journal of Pediatric Gastroenterology and Nutrition·Yuko Tazuke, Daniel H Teitelbaum
Dec 12, 1997·Alcoholism, Clinical and Experimental Research·H A JärveläinenK O Lindros
Apr 16, 1998·Gut·W D ClementsB J Rowlands
May 29, 1998·American Journal of Respiratory and Critical Care Medicine·A J LechnerG M Matuschak
Dec 22, 2010·Journal of Translational Medicine·Ming-Huei ChouTsun-Mei Lin
May 1, 2001·Seminars in Pediatric Surgery·D H Teitelbaum, T Tracy
Jan 1, 1996·Journal of Neuroimmunology·E GaleaD L Feinstein
Oct 16, 2004·Liver International : Official Journal of the International Association for the Study of the Liver·Katherine L LeicesterBruce R Bacon
Jun 10, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Katherine L LeicesterBruce R Bacon
Nov 14, 1997·The Journal of Surgical Research·E S FoxT F Tracy
Feb 6, 1998·The Journal of Laboratory and Clinical Medicine·J M BellezzoE S Fox
Mar 25, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G L SuS C Wang
Nov 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M E SewnathD J Gouma
Jul 18, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Grace L Su
Jan 14, 1999·The American Journal of Physiology·C A RichardD J Loegering
Mar 10, 1999·The American Journal of Physiology·K IkejimaR G Thurman

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