CD14 mediate endotoxin induction of nitric oxide synthase in cultured brain glial cells

Journal of Neuroimmunology
E GaleaD L Feinstein

Abstract

Here we show that lipopolysaccharide (LPS) induction of glial inducible nitric oxide synthase (iNOS) requires membrane (m) and soluble (s) forms of CD14. In glial cell cultures, an anti-rat CD14 monoclonal antibody detected CD14 protein in whole cells and cell lysates, and reduced LPS-dependent iNOS expression. Glial cells and normal brain tissue expressed CD14 mRNA, as revealed by isolation of a rat CD14 clone (rCD14) from an astrocyte cDNA library and RT-PCR analysis. Finally, serum the ED(50) of LPS required for glial iNOS expression, and antibodies against sCD14 blocked the potentiating effect of serum.

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E GaleaD J Reis
Dec 1, 1992·The Journal of Experimental Medicine·E A FreyS D Wright
Sep 1, 1992·Journal of Neurochemistry·M L Simmons, S Murphy
Aug 1, 1992·PCR Methods and Applications·E Galea, D L Feinstein
Apr 15, 1992·Cellular Immunology·J ZielasekH P Hartung
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P KubesD N Granger
May 31, 1991·Science·C R Maliszewski
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G GillilandH F Bunn
Dec 1, 1986·European Journal of Immunology·V BazilI Hilgert
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J G SutcliffeF E Bloom
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Jan 1, 1994·Journal of Neurochemistry·D L FeinsteinD J Reis
Jan 1, 1994·The Journal of Experimental Medicine·E HailmanS D Wright
Feb 15, 1994·Journal of Neuroscience Research·E GaleaD L Feinstein
Apr 1, 1995·The Journal of Experimental Medicine·R R Ingalls, D T Golenbock
May 5, 1995·The Journal of Biological Chemistry·P S TobiasR J Ulevitch
Jan 1, 1993·European Journal of Immunology·T EspevikA Sundan
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J PuginP S Tobias
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H KoprowskiB Dietzschold
Mar 1, 1993·Immunology Today·H W Ziegler-Heitbrock, R J Ulevitch
Aug 1, 1993·Trends in Neurosciences·S MurphyJ P Schwartz
Jul 1, 1993·The European Journal of Neuroscience·M L Simmons, S Murphy

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Citations

Jun 11, 2002·Journal of Molecular and Cellular Cardiology·Kou-ichi Tomaru KiMasahiko Kurabayashi
Feb 27, 2001·Neurochemistry International·B KalmárE Madarász
Nov 9, 2002·Journal of Neurochemistry·Thomas J MontineRichard M Breyer
Oct 29, 1998·Mediators of Inflammation·V A VincentA M Van Dam
Jun 15, 2006·Antioxidants & Redox Signaling·Ramendra N Saha, Kalipada Pahan
Dec 12, 1997·Alcoholism, Clinical and Experimental Research·H A JärveläinenK O Lindros
Oct 19, 2004·Clinical Microbiology Reviews·R Bryan RockPhillip K Peterson
Jul 11, 2013·Journal of Neuroinflammation·Ara E HinojosaJosé L M Madrigal
Mar 4, 2014·Journal of Neuroimmunology·Clas MalmeströmBob Olsson
Mar 10, 2001·Journal of Neuroimmunology·M T Heneka, D L Feinstein
Mar 22, 2008·FEMS Immunology and Medical Microbiology·Maria Antonietta PanaroVincenzo Mitolo
Oct 9, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Begoña M EscribanoIsaac Túnez
Aug 24, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M T HenekaH Wiesinger
Jul 13, 2013·Movement Disorders : Official Journal of the Movement Disorder Society·Bob OlssonHenrik Zetterberg
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M YinR G Thurman
Jun 22, 2002·Annals of the New York Academy of Sciences·Gwang-Ho JeohnJau-Shyong Hong
Jun 22, 2002·Annals of the New York Academy of Sciences·Cynthia L CooperJau-Shyong Hong
Aug 3, 2000·American Journal of Physiology. Heart and Circulatory Physiology·D B CowanF X McGowan
Jan 24, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chengjiang GaoPaul C Kuo
Oct 8, 1997·The American Journal of Physiology·R A SchroederP C Kuo
Mar 10, 1999·The American Journal of Physiology·K IkejimaR G Thurman
Jul 17, 1999·The American Journal of Physiology·T YangJ B Schnermann

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