CD14 promoter polymorphisms have no functional significance and are not associated with atopic phenotypes

Pharmacogenetics and Genomics
Xiao Hui LiangDe Yun Wang

Abstract

A polymorphism at CD14/-159 has been reported to be associated with atopic phenotypes in several studies. However, conflicting results from association studies in different populations have been reported. This study aimed to investigate the relationship between CD14 promoter polymorphisms and atopic phenotypes in Singaporean Chinese and the biological characterization of these polymorphisms. A total of 171 atopic and 160 non-atopic adult subjects were included and their serum soluble CD14 (sCD14) and total immunoglobulin E (tIgE) levels were measured. Screening of single nucleotide polymorphisms (SNPs) in the CD14 promoter was performed using direct PCR-sequencing and restriction fragment length polymorphism methods. The functional significance of SNPs was investigated using reporter assay system. Three previous reported SNPs (CD14/-159, -1145 and -1359) and a novel SNP (CD14/-550) were detected. Significant linkage disequilibrium was found among these four loci of CD14 gene. However, no significant difference was found in the genotype frequencies of these SNPs between non-atopy and atopy groups. Furthermore, no transcriptional activities of these SNPs were detected using reporter gene assay in three cell-lines (HepG-2, THP-1 a...Continue Reading

References

May 1, 1997·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·P G HoltB Björkstén
Oct 12, 1999·Clinical Genetics·P S GaoJ M Hopkin
Jan 13, 2000·Current Opinion in Immunology·M F Moffatt, W O Cookson
Sep 26, 2000·Allergy·K C Barnes
Sep 27, 2000·Human Molecular Genetics·W O Cookson, M F Moffatt
Nov 25, 2000·Toxicology·J R Lapa e SilvaB B Vargaftig
Mar 20, 2001·American Journal of Human Genetics·M StephensP Donnelly
Apr 3, 2001·American Journal of Respiratory and Critical Care Medicine·G H KoppelmanE R Bleecker
Apr 18, 2001·International Archives of Allergy and Immunology·D VercelliF Martinez
Nov 1, 2001·Respiratory Research·F D Martinez
Nov 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T D LeVanD Vercelli
Nov 15, 2001·The Journal of Allergy and Clinical Immunology·J BousquetUNKNOWN World Health Organization
Apr 26, 2002·Immunology·Masako Toda, Santa Jeremy Ono
Dec 31, 2002·Toxicology·David B Peden
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D Vercelli
Feb 13, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C SenglerUNKNOWN German Multicenter Allergy Study Group
Aug 5, 2003·The Journal of Allergy and Clinical Immunology·Natalija Novak, Thomas Bieber
Dec 4, 2003·European Journal of Immunogenetics : Official Journal of the British Society for Histocompatibility and Immunogenetics·A HeinzmannK A Deichmann
Jun 9, 2005·The Journal of Allergy and Clinical Immunology·April Zambelli-WeinerKathleen C Barnes
Jun 1, 1968·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W G Hill, A Robertson

❮ Previous
Next ❯

Citations

Sep 21, 2013·Cellular Immunology·Yousri M HusseinSaad S Alzahrani
Jan 23, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Chikako KiyoharaYoshihiro Miyake
Apr 10, 2014·Clinical Reviews in Allergy & Immunology·Zhanglei MuJianzhong Zhang
Dec 10, 2009·Journal of Gastroenterology·Atsushi MasamuneTooru Shimosegawa
Jan 23, 2016·Frontiers in Immunology·Priscila Saamara MaziniPaulo Rodrigues-Santos
Dec 17, 2008·The Journal of Investigative Dermatology·Anna De BenedettoLisa A Beck
Oct 24, 2007·The Journal of Allergy and Clinical Immunology·Tricia D LevanFernando D Martinez
Dec 1, 2012·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Monica Cheng Munthe-KaasKarin C Lødrup Carlsen
Apr 20, 2010·The Journal of Allergy and Clinical Immunology·Monica Cheng Munthe-KaasRobert Lyle
Jan 30, 2010·The Journal of Allergy and Clinical Immunology·Kathleen C Barnes
Aug 8, 2014·Frontiers in Immunology·Natalia Castaño-RodríguezHazel M Mitchell
Mar 18, 2017·Respiratory Research·Tricia D LeVanDebra J Romberger
Dec 28, 2017·PloS One·Magdalena DruszczynskaWieslawa Rudnicka
Jun 17, 2020·Journal of Evidence-based Medicine·Nipith CharoenngamPatompong Ungprasert
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anna Ferrer-AdmetllaFerran Casals
Apr 18, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Dan ZhaoDongxin Lin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atopic Dermatitis

Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.

Related Papers

International Journal of Pediatric Otorhinolaryngology
Hee Joon KangHeung-Man Lee
Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Dan ZhaoDongxin Lin
Allergology International : Official Journal of the Japanese Society of Allergology
Eduardo CamposYoichi Kohno
© 2021 Meta ULC. All rights reserved