CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation

Immunobiology
Sawitree ChiampanichayakulWatchara Kasinrerk

Abstract

CD147 is a leukocyte surface molecule which belongs to the immunoglobulin superfamily. It is broadly expressed on various cell types and is a lymphocyte activation-associated molecule. In order to study the function of CD147, five CD147 monoclonal antibodies (mAbs) were generated: M6-2F9; M6-1D4; M6-1F3; M6-1B9; and M6-1E9. Biochemical characterizations and cross-blocking experiments indicated that M6-1B9 and M6-1E9 recognize the same or contiguous epitopes on CD147. By employing COS transfectants expressing CD147 membrane-distal domain (domain 1) and membrane-proximal domain (domain 2), mAbs M6-2F9, M6-1D4, M6-1B9, and M6-1E9 were shown to recognize epitopes located on domain 1 of the molecule. Functional studies indicated that engagement of CD147 by mAbs M6-1B9 and M6-1E9 strongly inhibited lymphocyte proliferation induced by a CD3 mAb. In contrast, mAbs M6-2F9, M6-1D4, and M6-1F3 induced U937 homotypic cell aggregation. The results indicate that CD147 contains at least two bioactive domains. Epitopes responsible for induction of cell aggregation are different from those regulating lymphocyte activation.

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Citations

Jul 30, 2009·Cytotechnology·Napaporn ApiratmateekulWatchara Kasinrerk
Jan 30, 2008·BMC Biotechnology·Khajornsak TragoolpuaChatchai Tayapiwatana
Jul 29, 2008·Connective Tissue Research·Eric HuetSuzanne Menashi
Mar 30, 2010·Clinical and Experimental Immunology·V YurchenkoM Bukrinsky
Oct 10, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sumalee ObchoeiSopit Wongkham
May 15, 2013·Cellular Immunology·Johannes Landskron, Kjetil Taskén
Jan 22, 2015·Journal of Cardiovascular Translational Research·Cuiping WangGuohong Li
Feb 14, 2015·Immunobiology·Nuttapol ChruewkamlowSawitree Chiampanichayakul
Jun 3, 2006·International Immunology·Nutjeera IntasaiChatchai Tayapiwatana
Oct 14, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer N HahnV Wee Yong
Feb 6, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Verena SupperHannes Stockinger
May 16, 2015·Journal of Leukocyte Biology·Jennifer Nancy HahnV Wee Yong
Aug 24, 2021·Frontiers in Immunology·Supansa PataWatchara Kasinrerk

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