CD147 (EMMPRIN) controls malignant properties of breast cancer cells by interdependent signaling of Wnt and JAK/STAT pathways

Molecular and Cellular Biochemistry
Nadine KnuttiKarlheinz Friedrich

Abstract

EMMPRIN (extracellular matrix metalloproteinase inducer, EMN, CD147) is a member of the immunoglobulin superfamily expressed in numerous cell types both as a soluble and a membrane-spanning glycoprotein. It is involved in many physiological processes, as well as in cancer. This study addresses mechanisms of crosstalk between EMN-driven cancer-related cellular responses and the canonical Wnt-pathway in MCF-7 breast carcinoma cells. Genetic knockdown of EMN in MCF-7 resulted in characteristic changes in cellular shape, organization of the actin cytoskeleton and malignancy profile, indicating that EMN expression represses cell motility, but, in contrast, exerts a stimulatory effect on cell proliferation and invasive properties. Increased invasiveness coincided with elevated expression of Wnt-target genes and established invasion driver matrix metalloproteinase MMP14. Activation of the downstream Wnt-pathway by means of heterologous β-catenin and/or TCF-4 expression, through inhibition of GSK-3β by LiCl treatment, or by cell stimulation with insulin-like growth factor-1 (IGF-1) resulted in increased EMN expression. EMN over-expression raised the ratio of the two opposing Wnt pathway-driven transcription factors Sp1 and Sp5, leading...Continue Reading

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Citations

Aug 15, 2019·Cancer Biology & Therapy·Miao WangYu Li
Jul 31, 2020·Journal of Experimental & Clinical Cancer Research : CR·Shi-Jie WangZhi-Nan Chen
Nov 19, 2020·Patterns·M Hadi AminiPanos M Pardalos
Mar 25, 2019·Life Sciences·Dhivya KumarKrishna Kumar Subramanian

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Methods Mentioned

BETA
nuclear translocation
transfection
FCS
Assay
electrophoresis
fluorescence microscopy
flow cytometry

Software Mentioned

Cyflogic
TScratch
ALLGEN
SPSS
CASY R Cell
- PROMO - Software

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