CD147 overexpression on synoviocytes in rheumatoid arthritis enhances matrix metalloproteinase production and invasiveness of synoviocytes

Arthritis Research & Therapy
Ping ZhuZhi-nan Chen

Abstract

Macrophage-like synoviocytes and fibroblast-like synoviocytes (FLS) are known as the most active cells of rheumatoid arthritis (RA) and are close to the articular cartilage in a position enabling them to invade the cartilage. Macrophage-like synoviocytes and FLS expression of matrix metalloproteinases (MMPs) and their interaction has aroused great interest. The present article studied the expression of CD147, also called extracellular matrix metalloproteinase inducer, on monocytes/macrophages and FLS from RA patients and its potential role in enhancing MMPs and the invasiveness of synoviocytes. Expression of CD147 on FLS derived from RA patients and from osteoarthritis patients, and expression of CD147 on monocytes/macrophages from rheumatic synovial fluid and healthy peripheral blood were analyzed by flow cytometry. The levels of CD147, MMP-2 and MMP-9 mRNA in FLS were detected by RT-PCR. The role of CD147 in MMP production and the cells' invasiveness in vitro were studied by the co-culture of FLS with the human THP-1 cell line or monocytes/macrophages, by gel zymography and by invasion assay. The results showed that the expression of CD147 was higher on RA FLS than on osteoarthritis FLS and was higher on monocytes/macrophages...Continue Reading

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Citations

Apr 20, 2007·Arthritis Research & Therapy·Anette KnedlaUlf Müller-Ladner
Jun 2, 2007·Journal of Leukocyte Biology·Jesse M DamskerStephanie L Constant
Jan 7, 2014·International Journal of Biological Sciences·Hui YaoZhi-Nan Chen
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Methods Mentioned

BETA
PMA
flow cytometry
PCR
electrophoresis

Software Mentioned

Cell Quest
Image
SPSS

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