CD163 and CD206 expression does not correlate with tolerance and cytokine production in LPS-tolerant human monocytes

Cytometry. Part B, Clinical Cytometry
Amanda Barba Alves-JanuzziR Salomão

Abstract

Lipopolysaccharide (LPS)-tolerant monocytes produce small amounts of inflammatory cytokines, which is one of the characteristics of the alternative activated macrophages (AAM). These cells exhibited an increased expression of CD206 and CD163. Given the functional similarities of AAMs with the modulation of monocytes' functions observed during sepsis and LPS-tolerance, we evaluated whether the inhibition of inflammatory cytokine production by LPS-tolerant monocytes is associated with the phenotype of cells expressing CD206 and CD163. We investigated whether tolerant human monocytes would modulate their expression of CD206 and CD163, markers of alternative activation, and whether the level of their expression would be related to cytokines detection. Tolerance to LPS was induced in peripheral blood mononuclear cell by pre-incubating the cells with increasing concentrations of LPS. The expression of CD206 and CD163 and intracellular TNF-α and IL-6 was determined 24 h after LPS challenge by flow cytometry. No differences in CD163 expression were observed between tolerant and non-tolerant cells, while the expression of CD206, which was decreased following LPS stimulation in non-tolerized cells, was further reduced in tolerant cells. ...Continue Reading

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Citations

Apr 28, 2017·Cytometry. Part B, Clinical Cytometry·Claude Lambert, Ulrich Sack
Apr 18, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R SalomãoM K C Brunialti
Feb 14, 2020·Journal of Cellular Physiology·Sarah VakiliJay Rappaport
Feb 22, 2018·Clinical Chemistry and Laboratory Medicine : CCLM·Simon DegandtXavier Bossuyt
Mar 24, 2018·International Journal of Inflammation·Lusine KhondkaryanGayane Manukyan

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