PMID: 8958149Jan 1, 1997Paper

CD18/ICAM-1-dependent nitric oxide production of Kupffer cells as a cause of mitochondrial dysfunction in hepatoma cells: influence of chronic alcohol feeding

Free Radical Biology & Medicine
I KuroseH Ishii

Abstract

The present study was designed to monitor the process for hepatoma cell injury induced by Kupffer cells. The non-activated Kupffer cells isolated from male Wistar rats reduced the mitochondrial membrane potential in the cocultured AH70 cells, which was indicated by the decreased rhodamine 123 (Rh123) fluorescence. Increased level of nitrite and nitrate in the medium and induction of iNOS in Kupffer cells were observed after coculture with AH70 cells. Incubation with either NG-monomethyl-L-arginine or aminoguanidine attenuated the increased nitric oxide (NO) production of Kupffer cells and the decreased Rh123 fluorescence of AH70 cells. Fluo-3, a calcium-sensitive probe, fluorescence in Kupffer cells increased after coculture with AH70 cells. Addition of TMB-8, a calcium inhibitor, or monoclonal antibody directed against ICAM-1 or CD18 prevented the increases in fluo-3 fluorescence and NO production of Kupffer cells and Kupffer cell-induced mitochondrial dysfunction in AH70 cells, suggesting the involvement of calcium mobilization and CD18/ICAM-1. It is therefore suggested that the Kupffer cell-mediated mitochondrial dysfunction of hepatoma cells largely depends on NO production by iNOS, and that the NO production by Kupffer cel...Continue Reading

References

Jul 1, 1992·Acta Physiologica Scandinavica·S Moncada
Nov 1, 1992·Archives of Biochemistry and Biophysics·H IschiropoulosJ S Beckman
Nov 1, 1992·Archives of Biochemistry and Biophysics·J S BeckmanM Tsai
Aug 30, 1991·Biochemical and Biophysical Research Communications·M LepoivreM Fontecave
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J S BeckmanB A Freeman
Sep 11, 1990·European Journal of Biochemistry·K Decker
Jan 1, 1990·The Journal of Clinical Investigation·D L GrangerD T Durack
Sep 1, 1989·Biological Chemistry Hoppe-Seyler·G LatochaK Decker
Aug 30, 1989·Biochemical and Biophysical Research Communications·M Eberhard, P Erne
Nov 2, 1993·European Journal of Pharmacology·K HasanR G Tilton
Nov 1, 1993·British Journal of Pharmacology·M J GriffithsT W Evans
Sep 23, 1994·Cell·C Nathan, Q W Xie
Feb 1, 1994·Biological Chemistry Hoppe-Seyler·J S BeckmannC R White
Jun 1, 1993·Alcoholism, Clinical and Experimental Research·M H BasistaV J Dindzans
Jun 1, 1993·Alcoholism, Clinical and Experimental Research·B WatzlR R Watson
Aug 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I KuroseM Tsuchiya
Sep 5, 2002·Medical and Pediatric Oncology·Charles Stiller

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Citations

Jun 14, 2002·Alcohol·Abraham P Bautista
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P SartiM Brunori

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