Abstract
Ligation of the CD2 cell surface glycoprotein expressed on T lymphocytes and NK cells induces protein tyrosine phosphorylation and activation of the Src kinases, LCK and FYN. We show here that in Jurkat T leukemia cells and in peripheral blood T cells, CD2 stimulation also leads to tyrosine phosphorylation and activation of the Tec family kinase, EMT/ITK/TSK. Activation of EMT by CD2 was induced by mitogenic pairs of CD2 mAb, certain single CD2 mAb followed by secondary antibody cross-linking, and CD58-bearing sheep red blood cells. With the use of different Jurkat cell mutants it was demonstrated that CD2-mediated activation of EMT required expression of LCK, but not require surface expression of the CD3 zeta chain. Receptor-mediated activation of LCK does not in itself lead to activation of this Tec kinase since induction of LCK by ligation of CD4 or CD5 did not result in activation of EMT. The activation of EMT during CD2 signaling suggests an important role for this kinase in CD2 co-stimulation of T cell responses.
Citations
Aug 7, 2002·FEBS Letters·Shengli Hao, Avery August
Jan 17, 2002·Molecular Immunology·Hisanori UmeharaNaochika Domae
Jul 20, 2002·The International Journal of Biochemistry & Cell Biology·Avery AugustMelanie Ragin
Apr 5, 2001·Trends in Immunology·C D TsoukasT Kawakami
Jan 20, 1999·Immunology·D J GuyotM D Lairmore
Sep 1, 2009·The Journal of Biological Chemistry·Qian Qi, Avery August
Jun 13, 1998·The Journal of Experimental Medicine·K Q LiuL J Berg
Mar 18, 2005·Annual Review of Immunology·Leslie J BergPamela L Schwartzberg
Jul 13, 2011·Journal of Signal Transduction·Juris A Grasis, Constantine D Tsoukas
Jan 12, 2002·Peptides·V ChiţuE Monostori
Feb 27, 1999·Leukemia & Lymphoma·J KaukonenA Palotie
Oct 25, 2006·The Journal of Biological Chemistry·Qian QiAvery August
Oct 6, 1997·The Journal of Biological Chemistry·S D HeyeckL J Berg
Jan 5, 1999·The Journal of Biological Chemistry·W C YangD Olive
Sep 3, 1998·The Journal of Biological Chemistry·R Sunder-Plassmann, E L Reinherz
Sep 13, 2001·The Journal of Biological Chemistry·M P MartelliB E Bierer
May 29, 2001·The Journal of Biological Chemistry·H Yang, E L Reinherz
Dec 2, 1999·Immunologic Research·T ZellY Shimizu
Aug 26, 1998·Molecular and Cellular Biology·W J KivensY Shimizu
Sep 12, 2020·Current Drug Targets·Adekunle Babajide RowaiyeCharles Okechukwu Esimone
Aug 29, 2021·Viruses·Sabal ChaulagainDaniel L Rock