CD20 positive human B lymphocytes separated with the magnetic cell sorter (MACS) can be induced to proliferation and antibody secretion in vitro.

Journal of Immunological Methods
H AbtsH Tesch

Abstract

A method is described for the efficient purification of human B lymphocytes from peripheral blood by magnetic separation. Biotinylated, superparamagnetic particles were coupled to target cells by fluorescein isothiocyanate conjugated avidin and biotinylated monoclonal antibodies directed against cell surface antigens. This combination permitted flow cytometric control of the magnetic separation. Ficoll-Paque-separated peripheral blood mononuclear cells were first eliminated from monocytes by leucine-methyl ester treatment. B cells were enriched to 97% after magnetic depletion of CD3-positive T cells and magnetic enrichment of CD20-positive B cells. The separated B cells could be induced to proliferation and antibody production by various in vitro stimuli.

References

Jun 30, 1975·Annals of the New York Academy of Sciences·M R Loken, L A Herzenber
Jan 1, 1979·Journal of Immunological Methods·C GutierrezM Kreisler
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L J Wysocki, V L Sato
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E SmelandR Ohlsson
Aug 27, 1974·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C R Parish, J A Hayward
Apr 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·D J VolkmanA S Fauci

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Citations

May 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·J W BulteT H The
Jul 6, 1992·Journal of Immunological Methods·C Andreoni, M Delaage
Aug 26, 1993·Journal of Immunological Methods·J P Hancock, J T Kemshead
Oct 26, 1995·Journal of Immunological Methods·C A Ellison, J G Gartner
Dec 15, 1995·Journal of Immunological Methods·S J RothT A Springer
Jul 1, 1994·Journal of Biochemical and Biophysical Methods·S BarthH Schnabl
Apr 1, 1991·Current Opinion in Immunology·F L Battye, K Shortman
Jan 25, 2003·Veterinary Immunology and Immunopathology·A CrawleyB N Wilkie
Jun 12, 2003·Vaccine·A Crawley, B N Wilkie
Dec 1, 1996·Alcoholism, Clinical and Experimental Research·P W JareoM J Gentry
Sep 1, 1994·The Journal of Clinical Investigation·T G DiacovoT A Springer
Feb 20, 1999·Neuroscience Letters·K ShogeM Sasa
Dec 10, 2014·Cancer Biotherapy & Radiopharmaceuticals·Sandra L Pena LuengasEduardo Mansilla
Jun 16, 2010·Journal of Periodontal Research·B CalenicT Hirata
Jun 29, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Andreas Grützkau, Andreas Radbruch
Feb 1, 1993·Research in Immunology·N JacobsN Schaaf-Lafontaine
Mar 6, 2002·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Olaf Burkhardt, Hans-Joachim Merker
Apr 9, 2014·Nanomedicine : Nanotechnology, Biology, and Medicine·Daisuke KamiSatoshi Gojo
Feb 13, 2001·Blood Cells, Molecules & Diseases·T J WoodlockG B Segel
Aug 1, 1994·European Journal of Immunology·I AebischerB M Stadler
Sep 1, 1994·European Journal of Immunology·M R StämpfliB M Stadler
Apr 30, 2014·International Archives of Allergy and Immunology·Alina NeunkirchnerWinfried F Pickl
Feb 2, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Yaju ZhaoWeihong Tan
Jul 1, 2020·Scandinavian Journal of Immunology·Aicha LaghmouchiInge Jedema
Jan 1, 1997·Clinical and Diagnostic Laboratory Immunology·L GergelyV Agnello

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