CD200 expression in human cultured bone marrow mesenchymal stem cells is induced by pro-osteogenic and pro-inflammatory cues

Journal of Cellular and Molecular Medicine
Charalampos PontikoglouFrédéric Deschaseaux

Abstract

Similar to other adult tissue stem/progenitor cells, bone marrow mesenchymal stem/stromal cells (BM MSCs) exhibit heterogeneity at the phenotypic level and in terms of proliferation and differentiation potential. In this study such a heterogeneity was reflected by the CD200 protein. We thus characterized CD200(pos) cells sorted from whole BM MSC cultures and we investigated the molecular mechanisms regulating CD200 expression. After sorting, measurement of lineage markers showed that the osteoblastic genes RUNX2 and DLX5 were up-regulated in CD200(pos) cells compared to CD200(neg) fraction. At the functional level, CD200(pos) cells were prone to mineralize the extra-cellular matrix in vitro after sole addition of phosphates. In addition, osteogenic cues generated by bone morphogenetic protein 4 (BMP4) or BMP7 strongly induced CD200 expression. These data suggest that CD200 expression is related to commitment/differentiation towards the osteoblastic lineage. Immunohistochemistry of trephine bone marrow biopsies further corroborates the osteoblastic fate of CD200(pos) cells. However, when dexamethasone was used to direct osteogenic differentiation in vitro, CD200 was consistently down-regulated. As dexamethasone has anti-inflamma...Continue Reading

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Citations

Jan 25, 2018·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Mehdi NajarLaurence Lagneaux
Sep 25, 2019·Cells·Irina V KholodenkoKonstantin N Yarygin
Aug 28, 2020·Frontiers in Immunology·Siyu LiuShu Guo
Dec 2, 2020·European Journal of Dentistry·Alexander Patera NugrahaIgo Syaiful Ihsan
Aug 12, 2021·Nature Communications·D'Juan T FarmerStephen R F Twigg
Sep 2, 2021·Blood Advances·Paraskevi DiamantiAllison Blair

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Methods Mentioned

BETA
PCR
flow cytometry
biopsy
Protein Assay
Fluorescence

Software Mentioned

ImageJ
CellquestPro [UNK]
Chemocapt

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