CD200 Expression Marks a Population of Quiescent Limbal Epithelial Stem Cells with Holoclone Forming Ability

Stem Cells
Sanja BojicMajlinda Lako

Abstract

One of the main challenges in limbal stem cell (LSC) biology and transplantation is the lack of definitive cell surface markers which can be used to identify and enrich viable LSCs. In this study, expression of 361 cell surface proteins was assessed in ex vivo expanded limbal epithelial cells. One marker, CD200 was selected for further characterization based on expression in a small subset of limbal epithelial cells (2.25% ± 0.69%) and reduced expression through consecutive passaging and calcium induced differentiation. CD200 was localized to a small population of cells at the basal layer of the human and mouse limbal epithelium. CD200+ cells were slow cycling and contained the majority of side population (SP) and all the holoclone forming progenitors. CD200+ cells displayed higher expression of LSCs markers including PAX6, WNT7A, CDH3, CK14, CK15, and ABCB5 and lower expression of Ki67 when compared to CD200- . Downregulation of CD200 abrogated the ability of limbal epithelial cells to form holoclones, suggesting an important function for CD200 in the maintenance and/or self-renewal of LSCs. A second marker, CD109, which was expressed in 56.29% ± 13.96% of limbal epithelial cells, was also found to co-localize with ΔNp63 in bo...Continue Reading

References

Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y BarrandonH Green
Mar 29, 2000·Survey of Ophthalmology·H S Dua, A Azuara-Blanco
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·G PellegriniM De Luca
Oct 30, 2003·The Journal of Investigative Dermatology. Symposium Proceedings·Ralf PausTaisuke Ito
Oct 16, 2004·The Journal of Investigative Dermatology·Michael D RosenblumRobert L Truitt
Apr 19, 2005·The British Journal of Ophthalmology·H S DuaA Joseph
Jul 30, 2005·Experimental Eye Research·Ursula Schlötzer-Schrehardt, Friedrich E Kruse
Dec 16, 2005·The Journal of Investigative Dermatology·Hiroyuki Matsue
Dec 16, 2005·The Journal of Investigative Dermatology·Michael D RosenblumRobert L Truitt
Jan 3, 2006·Journal of Dermatological Science·Michael D RosenblumRobert L Truitt
Jan 6, 2006·The Journal of Clinical Investigation·Manabu OhyamaJonathan C Vogel
Jun 7, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kenneth W FinnsonAnie Philip
Oct 27, 2006·Investigative Ophthalmology & Visual Science·Satoru YoshidaKazuo Tsubota
Jan 2, 2007·Investigative Ophthalmology & Visual Science·Edwin C FigueiraDenis Wakefield
Jan 9, 2007·Journal of Dermatological Science·Manabu Ohyama
Jun 15, 2007·The Journal of Cell Biology·Vanessa BarbaroMichele De Luca
Dec 25, 2007·Biochemical and Biophysical Research Communications·Ryuhei HayashiKohji Nishida
Sep 18, 2008·The British Journal of Dermatology·K C MeyerR Paus
Aug 28, 2009·Investigative Ophthalmology & Visual Science·Ewa Anna Meyer-BlazejewskaUrsula Schlötzer-Schrehardt
Jan 30, 2010·Science·Linheng Li, Hans Clevers
Jun 25, 2010·The New England Journal of Medicine·Paolo RamaGraziella Pellegrini
Jun 21, 2011·Investigative Ophthalmology & Visual Science·Thuy T TruongSophie X Deng
May 23, 2012·Methods in Molecular Biology·Manabu Ohyama, Tetsuro Kobayashi
Aug 1, 2012·The American Journal of Pathology·Shinji MiiMasahide Takahashi
Nov 7, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Charles Osei-BempongMajlinda Lako
Jun 8, 2013·Investigative Ophthalmology & Visual Science·Yuen Kuen CheongWilliam Sun
Dec 12, 2013·Stem Cells Translational Medicine·Aaron W Joe, Sonia N Yeung
Jan 8, 2014·International Journal of Medical Sciences·Jun LiLanjuan Li
Jul 18, 2014·Nature·Bruce R KsanderNatasha Y Frank

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