CD200R1 agonist attenuates LPS-induced inflammatory response in human renal proximal tubular epithelial cells by regulating TLR4-MyD88-TAK1-mediated NF-κB and MAPK pathway

Biochemical and Biophysical Research Communications
Yan DingZhuwen Yi

Abstract

Previous studies have revealed the anti-inflammatory effect of CD200Fc, an agonist of CD200R1 in autoimmune disease. However, little is known about its anti-inflammatory effects in kidney diseases. The aim of this study is to assess the function of CD200Fc in regulating lipopolysaccharide (LPS)-induced inflammatory response in human renal proximal tubular epithelial cells (hRPTECs) and the possible mechanisms. LPS reduced the CD200R1 expression in hRPTECs, and this effect was attenuated by CD200Fc in a dose-dependent manner. In addition, CD200Fc inhibited LPS-induced expressions of TLR4 and its adapter molecule (MyD88 and phosphorylation of TAK1), and abolished its interactions with MyD88 or TAK1 in hRPTECs cells. CD200Fc also attenuated LPS-induced phosphorylation of IκB, NF-κB-P65 translocation to nucleus, and increased phosphorylation of ERK1/2, p38 and JNK in hRPTECs. Moreover, CD200Fc suppressed the LPS-induced release of pro-inflammatory mediators in hRPTECs, including IL-1β, IL-6, IL-8, MCP-1, VCAM-1, ICAM-1, TNF-α, INF-α and INF-γ. Our results suggested that CD200Fc could inhibit the TLR4-mediated inflammatory response in LPS-induced hRPTECs, thus might be beneficial for the treatment of renal disease, such as lupus nep...Continue Reading

References

Mar 1, 1983·The Journal of Experimental Medicine·L HangF J Dixon
Dec 1, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·R M GorczynskiJ Hu
Mar 19, 2005·Nephron. Experimental Nephrology·Nicoletta RondaCarlo Buzio
Apr 26, 2007·The American Journal of Pathology·Tanuja ChitnisSamia J Khoury
Feb 12, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yingru LiuTanuja Chitnis
Nov 3, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michele AndreucciAshour Michael

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Citations

Mar 10, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Li JiangMin Li
Aug 22, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Kouhei OhnoTetsuji Miura
Aug 9, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Yan ChenJinjun Wang
Oct 1, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Xuliang HaoLei Chen
May 26, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yan XuWei Zhang

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