CD24 associates with EGFR and supports EGF/EGFR signaling via RhoA in gastric cancer cells

Journal of Translational Medicine
Wenjie DengJun Du

Abstract

CD24, a mucin-like membrane glycoprotein, plays a critical role in carcinogenesis, but its role in human gastric cancer and the underlying mechanism remains undefined. The contents of CD24 and epidermal growth factor receptor (EGFR) in gastric cancer cells (SGC-7901 and BGC-823) and non-malignant gastric epithelial cells (GES-1) were evaluated by Western blotting assay. Cellular EGFR staining was examined by immunofluorescence assay. Cell migration rate was measured by wound healing assay. The effects of depletion/overexperssion of CD24 on EGFR expression and activation of EGF/EGFR singaling pathways were evaluated by immunofluorescence, qPCR, Western blotting and flow cytometry techniques. RhoA activity was assessed by pulldown assay. CD24 and EGFR expression patterns in human gastric tumor samples were also investigated by immunohistochemistry staining. CD24 was overexpressed in human gastric cancer cells. Ectopic expression of CD24 in gastric epithelial cells augmented the expression of EGFR, while knockdown of CD24 in gastric cancer cells decreased the level of EGFR and cell migration velocity. To further explore the mechanisms, we investigated the effect of CD24 expression on EGF/EGFR signaling. We noticed that this effect...Continue Reading

References

May 25, 1995·Biochemical and Biophysical Research Communications·K SatoY Fukami
Jul 11, 1996·Nature·C LamazeS L Schmid
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Jul 21, 2004·The Journal of Cell Biology·Genkichi IzumiYoshimi Takai
Sep 2, 2004·Journal of Molecular Histology·G KristiansenP Altevogt
Sep 17, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Takako KanekoKozo Kaibuchi
Dec 7, 2005·International Journal of Cancer. Journal International Du Cancer·Kyung-Jae LeeUNKNOWN JPHC Study Group
Jan 5, 2006·Journal of Cell Science·Heidi SchabathPeter Altevogt
Aug 8, 2007·Annals of Surgical Oncology·Yuh-Yu ChouRay-Hwang Yuan
Sep 22, 2007·Drugs·Michael F Press, Heinz-Josef Lenz
Oct 1, 2004·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Nevine S DarwishWoo Ho Kim
Jul 9, 2010·American Journal of Physiology. Renal Physiology·Neal X ChenSharon M Moe
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Sep 2, 2011·Journal of Cancer Research and Clinical Oncology·Chaojun ZhangHua Yang
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Leiliane P SousaJoseph Schlessinger
May 10, 2012·The Journal of Clinical Endocrinology and Metabolism·Jung-Chien ChengPeter C K Leung
Jun 23, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jeffrey B KingSusan J Henning
Jul 5, 2012·Cellular and Molecular Life Sciences : CMLS·Niko P BretzPeter Altevogt
Aug 18, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·C A DuckworthD M Pritchard
Sep 27, 2012·Science Signaling·Nancy L Lill, Nurettin Ilter Sever
Jan 24, 2013·Breast Cancer Research : BCR·Claire Médale-GiamarchiGilles Favre
Feb 15, 2013·PloS One·Claudia A DumitruStephan Lang
Mar 30, 2013·International Journal of Molecular Sciences·Xiaojuan ChiJi-Long Chen
Dec 12, 2013·Apoptosis : an International Journal on Programmed Cell Death·Ying-Chao WangJing-Yuan Fang

❮ Previous
Next ❯

Citations

Oct 15, 2018·Journal of Cellular Physiology·Vahideh TarhrizShirin Eyvazi
Dec 5, 2018·Journal of Biochemical and Molecular Toxicology·Xiao-Yan Wu, Xue-Yan Yu
Oct 6, 2017·BMC Complementary and Alternative Medicine·Qin JiangQi Zhang
Aug 14, 2020·International Journal of Cancer. Journal International Du Cancer·Peter AltevogtGlen Kristiansen
Sep 6, 2018·Therapeutic Advances in Medical Oncology·Estefania Carrasco-GarciaAnder Matheu
Oct 16, 2016·Pathology Oncology Research : POR·Deniz ArikÖzgül Paşaoğlu
Oct 19, 2019·Cell Death & Disease·Jun-Peng DongFu Yang
Apr 30, 2019·Journal of Cellular and Molecular Medicine·Pengxiang MinJun Du
Jun 25, 2020·Current Gene Therapy·Yang-Hong NiWei Wang
Nov 17, 2020·Therapeutic Advances in Medical Oncology·Fadi E El RamiGebran W Khneizer
Aug 8, 2021·International Journal of Molecular Sciences·Amanda ChantziouPanayiotis A Theodoropoulos
Oct 17, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·YingYing HeDavid S Newburg

❮ Previous
Next ❯

Methods Mentioned

BETA
Transfection
scraping
coimmunoprecipitation
co-immunoprecipitation
PCR
flow cytometry
pulldown
GTPase
GTPases
immunoprecipitation

Software Mentioned

SPSS
StepOne

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.