CD24 cross-linking induces apoptosis in, and inhibits migration of, MCF-7 breast cancer cells.

BMC Cancer
Jong Bin KimDong-Young Noh

Abstract

The biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established. We examined the impact of CD24 cross-linking on human breast cancer cell line MCF-7. MCF-7 and MDA-MB-231 cells were treated with anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibodies to induce cross-linking, and then growth was studied. Changes in cell characteristics such as cell cycle modulation, cell death, survival in three-dimensional cultures, adhesion, and migration ability were assayed after CD24 cross-linking in MCF-7. Expression of CD24 was analyzed by flow cytometry in MDA-MB-231 and MCF-7 cells where 2% and 66% expression frequencies were observed, respectively. CD24 cross-linking resulted in time-dependent proliferation reduction in MCF-7 cells, but no reduction in MDA-MB-231 cells. MCF-7 cell survival was reduced by 15% in three-dimensional culture after CD24 cross-linking. Increased MCF-7 cell apoptosis was observed after CD24 cross-linking, but no cell cycle arrest was observed in that condition. The migration capacity of MCF-7 cells was diminished by 30% after CD24 cross-linking. Our results showed that CD24 cross-linking induced apoptosis and inhibited migration in MCF-7 breast cance...Continue Reading

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Citations

Feb 24, 2010·Pathology Oncology Research : POR·Sibel BektasSukru Oguz Ozdamar
Jul 21, 2010·Wiener klinische Wochenschrift·Heidrun KarlicPeter Valent
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Apr 18, 2015·Journal of Hematology & Oncology·Axel SchulenburgPeter Valent
Jun 25, 2020·Current Gene Therapy·Yang-Hong NiWei Wang
Jun 20, 2021·Molecular Biology Reports·Xinjian QuWen Wen

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Methods Mentioned

BETA
glycosylation
flow cytometry
Flow
FACS

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