CD24a expression levels discriminate Langerhans cells from dermal dendritic cells in murine skin and lymph nodes

The Journal of Investigative Dermatology
Susanne StutteIrmgard Förster

Abstract

Langerhans cells (LCs) and dermal dendritic cells (dDCs) are the professional antigen-presenting cells of the skin. Recently, their immunogenic versus tolerogenic role has come under re-investigation. LCs are distinguished from dDCs by Langerin (CD207) staining or by detection of Birbeck granules. However, for in vitro experiments it is desirable to have a simple and robust flow cytometric demarcation of both cell types. We show here that CD24a is expressed on LCs but not on dDCs isolated directly from the skin. Moreover, in combination with major histocompatibility complex class II (MHCII), CD24a expression levels distinguish LCs from dDCs in skin-draining lymph nodes after antigen activation and migration. High expression of CD24a correlated strictly with CD207 expression. MHCII(high) cells were unique for skin-draining lymph nodes and were shown to be the only cells carrying antigen after FITC painting of the skin. CD24a expression levels further differentiated LCs and dDCs in the MHCII(high) population. As staining for CD24a does not require fixation of cells, CD24a-stained cells can be used for in vitro experiments to analyze and compare the functional roles and properties of dDCs and LCs.

References

Jan 25, 2003·The Journal of Investigative Dermatology·Patrizia StoitznerNikolaus Romani
Jul 24, 2003·Nature Reviews. Immunology·Carlos Ardavín
Sep 17, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Nikolaus RomaniPatrizia Stoitzner
May 18, 2005·The Journal of Cell Biology·Clare L BennettBjörn E Clausen
Jun 15, 2005·Seminars in Immunology·Jenny Valladeau, Sem Saeland
Dec 17, 2005·Immunity·Daniel H KaplanMark J Shlomchik

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Citations

Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Susanne StutteIrmgard Förster
Feb 11, 2015·Clinical Reviews in Allergy & Immunology·Yixin TanQianjin Lu
Oct 23, 2009·Transplantation Reviews·Yue-Harn Ng, Geetha Chalasani
Sep 19, 2008·Immunity·David AlvarezUlrich H von Andrian
Mar 31, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Catherine HervouetFabienne Anjuère
Dec 5, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nancy LuckashenakThomas Brocker
Oct 22, 2019·European Journal of Immunology·Andrea CossarizzaArturo Zychlinsky
Sep 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Atsushi FukunagaStephen E Ullrich
Aug 10, 2019·Frontiers in Immunology·David PejoskiClaire-Anne Siegrist
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bettina JuxCharlotte Esser

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