CD271-selected mesenchymal stem cells from adipose tissue enhance cartilage repair and are less angiogenic than plastic adherent mesenchymal stem cells

Scientific Reports
Nupur KohliWilliam E B Johnson

Abstract

CD271 is a marker of bone marrow MSCs with enhanced differentiation capacity for bone or cartilage repair. However, the nature of CD271+ MSCs from adipose tissue (AT) is less well understood. Here, we investigated the differentiation, wound healing and angiogenic capacity of plastic adherent MSCs (PA MSCs) versus CD271+ MSCs from AT. There was no difference in the extent to which PA MSCs and CD271+ MSCs formed osteoblasts, adipocytes or chondrocytes in vitro. In contrast, CD271+ MSCs transplanted into athymic rats significantly enhanced osteochondral wound healing with reduced vascularisation in the repair tissue compared to PA MSCs and control animals; there was little histological evidence of mature articular cartilage formation in all animals. Conditioned medium from CD271+ MSC cultures was less angiogenic than PA MSC conditioned medium, and had little effect on endothelial cell migration or endothelial tubule formation in vitro. The low angiogenic activity of CD271+ MSCs and improved early stage tissue repair of osteochondral lesions when transplanted, along with a comparable differentiation capacity along mesenchymal lineages when induced, suggests that these selected cells are a better candidate than PA MSCs for the repai...Continue Reading

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Citations

Sep 6, 2020·Molecular Biology Reports·Anupama KakkarSiddharth Pandey
Sep 7, 2020·Journal of Cellular Physiology·Milad MoloudizargariMohammad Abdollahi
Jul 28, 2020·Materials·Diana Aparecida Dias CâmaraNelson Foresto Lizier
Feb 26, 2021·Stem Cells International·Shuangpeng JiangQuanyi Guo
Apr 19, 2021·Journal of Translational Medicine·Nerea Cuesta-GomezJohn D M Campbell

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Methods Mentioned

BETA
FCS
dissecting
scanning electron microscopy

Software Mentioned

Cell IQ analyser
Cell IQ
GraphPad Prism6
GraphPad

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