CD28 expression on T cell subsets in vivo and CD28-mediated T cell response in vitro in patients with rheumatoid arthritis

Arthritis and Rheumatism
P P SfikakisH Choremi-Papadopoulou

Abstract

In view of the critical importance of the CD28-CD80 (B7/BB1) costimulatory pathway in antigen-specific T cell activation and clonal expansion, we examined CD28 surface molecule expression in vivo, and T cell receptor/CD3-mediated and B7/BB1-costimulated T cell proliferation in vitro, in rheumatoid arthritis (RA). Two-color immunofluorescence analyses of peripheral blood and synovial fluid-derived T cells, as well as 3H-thymidine incorporation assays, were performed. In the peripheral blood of 31 patients with active, untreated RA, a mean of 91% (range 48-100%) of CD4+ and 46% (range 13-82%) of CD8+ T cell subsets were CD28+, which was not significantly lower than normal. Although an overall decrease in the number of T cells was not observed, the numbers of CD28+CD8+ T cells were significantly lower in RA patients (mean 233/microliters, versus 292/microliters in controls), and this decrease was more pronounced in patients with severe disease (mean 172/microliters). CD28 expression on peripheral CD8+ T cells in RA patients, but not in healthy individuals, correlated inversely with T cell activation as assessed by HLA-DR antigen expression. In contrast to the peripheral blood, RA synovial fluid T cells were almost exclusively CD28...Continue Reading

References

Feb 1, 1990·Immunology Today·C T Weaver, E R Unanue
May 3, 1990·The New England Journal of Medicine·E D Harris
Aug 26, 1994·Science·B K FinckD Wofsy
Feb 3, 1994·Nature·E A Clark, J A Ledbetter

❮ Previous
Next ❯

Citations

Dec 4, 1998·Springer Seminars in Immunopathology·N Oppenheimer-Marks, P E Lipsky
Sep 2, 1998·Immunologic Research·S N LiossisG C Tsokos
Dec 22, 1999·The Journal of Clinical Investigation·J TrambleyC P Larsen
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L Rifas, L V Avioli
Feb 6, 2007·Clinical Immunology : the Official Journal of the Clinical Immunology Society·P P SfikakisA N Theofilopoulos
Jun 14, 2005·European Journal of Clinical Investigation·P P SfikakisM A Dimopoulos
Aug 22, 2003·Clinical and Experimental Immunology·S WerwitzkeT Witte
Apr 15, 2008·Scandinavian Journal of Immunology·S WerwitzkeT Witte
Apr 25, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·L I Salazar-FontanaM Alvarez-Mon
Feb 13, 2003·British Journal of Haematology·Ourania KatopodisPetros P Sfikakis
Dec 22, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·L van de CorputJ C Kluin-Nelemans
Feb 1, 2000·Baillière's Best Practice & Research. Clinical Rheumatology·F C Breedveld
Mar 10, 2018·Nature Reviews. Immunology·Theo van den BroekFemke van Wijk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.