CD3 bright lymphocyte population reveal gammadelta T cells

Cytometry. Part B, Clinical Cytometry
Claude Lambert, Christian Genin

Abstract

In routine CD3/CD4/CD8 T-cell analysis, a CD3 bright population of lymphocytes is frequently observed. The aim of the present study was to identify the immunological significance of such CD3 bright lymphocytes. We analyzed samples from 31 healthy adult volunteers, 78 human immunodeficiency virus (HIV)-positive, and 78 renal transplanted patients. A clearly distinct CD3 bright (frequently CD4-/CD8-) T-cell fraction was observed in 84% of donors and was directly correlated with the fraction of gammadelta T cells (r2 = 0.64). CD3 overexpression on gammadelta T cells was confirmed by a combination of monoclonal antibody staining (CD3-ECD, gammadeltaTCR-FITC, and alphabetaTCR-PE-Cy5) or immunomagnetic purification of gammadelta T cells (i.e., MdFI 20 vs 8.86). The gammadelta T cells expressed CD8 polypeptide chains (alpha and beta) in all possible combinations. The largest proportion, surprisingly, were cells expressing CD8betabeta homodimers (43.8 +/- 16.5%). CD8alphaalpha homodimers were expressed on 14.2% (+/- 12.3) of total gammadelta T cells, whereas CD8alphabeta heterodimers were expressed on 12.2% (+/- 7.5). We also observed a bimodal distribution of the intensity of CD3 fluorescence of gammadelta T cells in immunocompromised...Continue Reading

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Citations

Sep 23, 2010·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Howard J Meyerson
Dec 15, 2005·Cytometry. Part B, Clinical Cytometry·Claude LambertGenin Christian
Oct 17, 2009·Cytometry. Part B, Clinical Cytometry·Fatima-Zahra El HentatiClaude Lambert
Apr 27, 2013·International Reviews of Immunology·Leonardo M R Ferreira
Jan 17, 2008·Blood·Fiona E Craig, Kenneth A Foon
Apr 19, 2019·PLoS Pathogens·Kyong-Mi ChangUNKNOWN Hepatitis B Research Network (HBRN)
Feb 3, 2016·American Journal of Reproductive Immunology : AJRI·Han LuLi-Ping Jin
Jul 12, 2018·Transplantation·Oliver McCallionFadi Issa
Aug 15, 2012·Immunological Reviews·Barbara S NikolajczykGerald V Denis
Nov 4, 2015·Cytometry. Part B, Clinical Cytometry·Alicia M HuntFiona E Craig
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stoyan DimitrovJan Born
Nov 4, 2008·Archives of Pathology & Laboratory Medicine·Anja C RodenCurtis A Hanson
Sep 1, 2021·Pharmaceutical Research·Huimin LiNing Zhang

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