CD3ε Expression Defines Functionally Distinct Subsets of Vδ1 T Cells in Patients With Human Immunodeficiency Virus Infection

Frontiers in Immunology
Pádraic J DunneDerek G Doherty

Abstract

Human γδ T cells expressing the Vδ1 T cell receptor (TCR) recognize self and microbial antigens and stress-inducible molecules in a major histocompatibility complex-unrestricted manner and are an important source of innate interleukin (IL)-17. Vδ1 T cells are expanded in the circulation and intestines of patients with human immunodeficiency virus (HIV) infection. In this study, we show that patients with HIV have elevated frequencies, but not absolute numbers, of circulating Vδ1 T cells compared to control subjects. This increase was most striking in the patients with Candida albicans co-infection. Using flow cytometry and confocal microscopy, we identify two populations of Vδ1 T cells, based on low and high expression of the ε chain of the CD3 protein complex responsible for transducing TCR-mediated signals (denoted CD3εlo and CD3εhi Vδ1 T cells). Both Vδ1 T cell populations expressed the CD3 ζ-chain, also used for TCR signaling. Using lines of Vδ1 T cells generated from healthy donors, we show that CD3ε can be transiently downregulated by activation but that its expression is restored over time in culture in the presence of exogenous IL-2. Compared to CD3εhi Vδ1 T cells, CD3εlo Vδ1 T cells more frequently expressed terminally...Continue Reading

References

Jan 1, 1994·Cellular Immunology·C AgostiniG Semenzato
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J B DeJarnetteP E Love
May 20, 1999·The Journal of Clinical Investigation·J DéchanetJ F Moreau
Mar 23, 2000·The Journal of Experimental Medicine·F M SpadaM B Brenner
Jul 18, 2002·Immunity·Sandra M Hayes, Paul E Love
Aug 6, 2003·The Journal of Experimental Medicine·Francesco DieliAlfredo Salerno
Sep 7, 2006·The Journal of Experimental Medicine·Constantinos PetrovasRichard A Koup
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pankaj TailorPere Santamaria
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dong-Ming KuangLimin Zheng
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jean-Francois BrodeurVibhuti P Dave
Jun 12, 2010·Nature Reviews. Immunology·Marc BonnevilleWilli K Born
May 12, 2011·Cellular and Molecular Life Sciences : CMLS·Daniela WeschDieter Kabelitz
Jan 19, 2012·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Hirohiko KurodaMasahide Ikeguchi
Jul 26, 2012·European Journal of Immunology·Li BaiAlbert Bendelac
Oct 11, 2012·Immunological Reviews·Michael S Kuhns, Hemant B Badgandi
Jan 26, 2013·Nature Reviews. Immunology·Pierre Vantourout, Adrian Hayday
Apr 3, 2013·The Journal of Infectious Diseases·Philip HartjenJulian Schulze zur Wiesch
Jun 7, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Bozgana A ManganDerek G Doherty
Jul 3, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Antoine RouxVictor Appay
Oct 1, 2013·Nature Immunology·Adam P UldrichDale I Godfrey
Jan 7, 2014·Annual Review of Immunology·Yueh-hsiu ChienMarc Bonneville

❮ Previous
Next ❯

Citations

Jul 22, 2020·Immunological Reviews·Elena Lo PrestiSerena Meraviglia
Jul 20, 2019·Clinical & Translational Immunology·Jennifer A Juno, Emily M Eriksson
Mar 19, 2021·Frontiers in Immunology·Cong QiJingxia Zhao
Apr 26, 2021·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Margaret R DunneThomas R Rogers

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
Flow Cytometry
confocal microscopy

Software Mentioned

GraphPad
FlowJo
Prism
Zen

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.