CD32-Expressing CD4 T Cells Are Phenotypically Diverse and Can Contain Proviral HIV DNA

Frontiers in Immunology
Genevieve E MartinJohn Frater

Abstract

Efforts to both characterize and eradicate the HIV reservoir have been limited by the rarity of latently infected cells and the absence of a specific denoting biomarker. CD32a (FcγRIIa) has been proposed to be a marker for an enriched CD4 T cell HIV reservoir, but this finding remains controversial. Here, we explore the expression of CD32 on CD3+CD4+ cells in participants from two primary HIV infection studies and identify at least three distinct phenotypes (CD32low, CD32+CD14+, and CD32high). Of note, CD4 negative enrichment kits remove the majority of CD4+CD32+ T cells, potentially skewing subsequent analyses if used. CD32high CD4 T cells had higher levels of HLA-DR and HIV co-receptor expression than other subsets, compatible with their being more susceptible to infection. Surprisingly, they also expressed high levels of CD20, TCRαβ, IgD, and IgM (but not IgG), markers for both T cells and naïve B cells. Compared with other populations, CD32low cells had a more differentiated memory phenotype and high levels of immune checkpoint receptors, programmed death receptor-1 (PD-1), Tim-3, and TIGIT. Within all three CD3+CD4+CD32+ phenotypes, cells could be identified in infected participants, which contained HIV DNA. CD32 expressio...Continue Reading

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Citations

Sep 23, 2020·PloS One·Amber K VirdiLena Al-Harthi
Jun 20, 2018·Frontiers in Immunology·Sixtine CoindreUNKNOWN ANRS CO6 PRIMO Cohort
Aug 24, 2019·Frontiers in Immunology·Chansavath PhetsouphanhJohn Frater
Dec 18, 2018·Frontiers in Immunology·María Pía HolgadoLourdes Arruvito
Oct 17, 2019·Frontiers in Immunology·Gilles DarcisAlexander O Pasternak
Jan 31, 2020·Science Translational Medicine·Kyungyoon J KwonRobert F Siliciano
Sep 21, 2018·Nature·Benjamin DescoursMonsef Benkirane
Apr 23, 2020·Frontiers in Cellular and Infection Microbiology·Jordan ThomasGeorgios Pollakis
Oct 9, 2020·Nature·Stylianos BournazosJeffrey V Ravetch
Feb 23, 2020·Cell Reports·Gilles DarcisAlexander O Pasternak

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Methods Mentioned

BETA
PCR
FACS
RNA assay
flow cytometry

Software Mentioned

SPARTAC
GraphPad Prism
HEATHER
R

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