Abstract
A 96-well-based suspension culture system for human hematopoietic progenitor cells has been developed to monitor the commitment and differentiation of CD34+ cells to specific lineages and the maintenance and expansion of CD34+ cells in vitro. The expression of maturation and lineage markers on the cells in culture was measured by enzyme-linked immunosorbent assay (ELISA). CD34+ cells were isolated from umbilical cord blood and fetal liver (90% purity) and were grown in liquid culture in 96-well plates for 10 days. The cells were then fixed with a glutaraldehyde-paraformaldehyde mixture, attaching the cells firmly to the plastic. An ELISA was performed, using appropriate primary antibodies directed against cell surface markers. The expression of four different lineage markers was measured: CD14 (monocyte), CD15 (neutrophil), platelet glycoprotein (GP) IIb/IIIa (CD41a, megakaryocyte) and glycophorin A (erythroid). The two-growth factor combination of interleukin 3 (IL-3) and stem cell factor (SCF) stimulated expression of CD14, CD15 and GP IIb/IIIa. Lineage-restricted growth factors such as erythropoietin (EPO), in combination with SCF, stimulated expression of glycophorin A. The three-factor combination of IL-3, SCF and EPO stim...Continue Reading
References
Sep 21, 1990·Science·S D WrightJ C Mathison
Nov 2, 1990·Cell·E LarsenB Furie
Nov 1, 1990·Transplantation·J L PlattF H Bach
Jul 1, 1987·International Journal of Cell Cloning·C I Civin, M R Loken
Jul 1, 1994·The Journal of Experimental Medicine·A BárcenaH Spits
Sep 1, 1993·The Journal of Experimental Medicine·P M LansdorpH Mayani
Sep 1, 1993·Stem Cells·R M Lemoli, S C Gulati
Jun 16, 1994·Nature·F J de SauvageW J Kuang
Jan 1, 1993·Stem Cells·S GulatiC Van-Poznak
Citations
Apr 1, 1999·Biotechnology and Bioengineering·P C CollinsE T Papoutsakis
Aug 30, 2003·Toxicology and Applied Pharmacology·James L GardnerEvan P Gallagher
Feb 28, 2002·Gene·Jiachang ZhangGriffin P Rodgers
Sep 21, 2000·British Journal of Haematology·Y FurukawaM Matsuda
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D S PereiraJ E Dick
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A RazatosG Georgiou
Jul 17, 2001·Journal of Hematotherapy & Stem Cell Research·P H ShawM Kletzel
Dec 1, 1996·Journal of Hematotherapy·M J SchneidkrautC C Huntenburg
Apr 1, 1997·Journal of Hematotherapy·C De BruynP Stryckmans
Sep 29, 2000·Stem Cells·G N SchwartzR E Gress
May 1, 1996·Stem Cells·G N SchwartzJ F Perdue
Jan 1, 1996·Stem Cells·M K WarrenG N Schwartz
Jan 1, 1997·Stem Cells·J A LaIuppaW M Miller
Jun 1, 1997·Baillière's Clinical Haematology·C L Sawyers
Dec 14, 2004·Biochemical Pharmacology·Craig G Moneypenny, Evan P Gallagher
Mar 4, 2006·Journal of Biotechnology·Yang LiuZhanfeng Cui
May 12, 2009·Biochemical Pharmacology·Seung Hyun KimTae Sung Kim
Jun 19, 2015·International Journal of Surgical Pathology·Jia ShenAaron W James
Oct 6, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jing ShaoEvan P Gallagher
Jan 31, 2009·Cell Transplantation·A A GaloyanA V Revishchin
Aug 23, 2008·Neurologia Medico-chirurgica·Fumiyuki MommaMasaharu Amagasa
Jan 31, 2012·Biomedical Materials·Shahram GhanaatiCharles James Kirkpatrick
Dec 7, 2002·Blood·Niro InabaHisashi Narimatsu
May 16, 2002·Blood·Hilit GurYair Reisner
Apr 25, 2017·The Journal of Clinical Investigation·Bon Ham YipJacqueline Boultwood
Aug 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M E MerinoC L Mackall
Jan 15, 2002·Journal of Biomolecular Screening·D E GreenwaltI Manchel
Jun 12, 2003·Annals of the New York Academy of Sciences·Yair ReisnerEsther Bachar-Lustig
Jan 13, 1998·Biochimica Et Biophysica Acta·A B RaitanoC L Sawyers