CD34+ cell expansion and expression of lineage markers during liquid culture of human progenitor cells

Stem Cells
M K WarrenJ Cone

Abstract

A 96-well-based suspension culture system for human hematopoietic progenitor cells has been developed to monitor the commitment and differentiation of CD34+ cells to specific lineages and the maintenance and expansion of CD34+ cells in vitro. The expression of maturation and lineage markers on the cells in culture was measured by enzyme-linked immunosorbent assay (ELISA). CD34+ cells were isolated from umbilical cord blood and fetal liver (90% purity) and were grown in liquid culture in 96-well plates for 10 days. The cells were then fixed with a glutaraldehyde-paraformaldehyde mixture, attaching the cells firmly to the plastic. An ELISA was performed, using appropriate primary antibodies directed against cell surface markers. The expression of four different lineage markers was measured: CD14 (monocyte), CD15 (neutrophil), platelet glycoprotein (GP) IIb/IIIa (CD41a, megakaryocyte) and glycophorin A (erythroid). The two-growth factor combination of interleukin 3 (IL-3) and stem cell factor (SCF) stimulated expression of CD14, CD15 and GP IIb/IIIa. Lineage-restricted growth factors such as erythropoietin (EPO), in combination with SCF, stimulated expression of glycophorin A. The three-factor combination of IL-3, SCF and EPO stim...Continue Reading

Citations

Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D S PereiraJ E Dick
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A RazatosG Georgiou
Apr 1, 1997·Journal of Hematotherapy·C De BruynP Stryckmans
Jun 1, 1997·Baillière's Clinical Haematology·C L Sawyers
Jun 19, 2015·International Journal of Surgical Pathology·Jia ShenAaron W James
Oct 6, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jing ShaoEvan P Gallagher
Apr 25, 2017·The Journal of Clinical Investigation·Bon Ham YipJacqueline Boultwood
Aug 16, 2001·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M E MerinoC L Mackall
Jan 15, 2002·Journal of Biomolecular Screening·D E GreenwaltI Manchel
Jun 12, 2003·Annals of the New York Academy of Sciences·Yair ReisnerEsther Bachar-Lustig
Jan 13, 1998·Biochimica Et Biophysica Acta·A B RaitanoC L Sawyers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
K Antman
© 2021 Meta ULC. All rights reserved