CD34-derived dendritic cells transfected ex vivo with HIV-Gag mRNA induce polyfunctional T-cell responses in nonhuman primates.

European Journal of Immunology
Gabrielle RomainFrédéric Martinon

Abstract

The pivotal role of DCs in initiating immune responses led to their use as vaccine vectors. However, the relationship between DC subsets involved in antigen presentation and the type of elicited immune responses underlined the need for the characterization of the DCs generated in vitro. The phenotypes of tissue-derived APCs from a cynomolgus macaque model for human vaccine development were compared with ex vivo-derived DCs. Monocyte/macrophages predominated in bone marrow (BM) and blood. Myeloid DCs (mDCs) were present in all tested tissues and were more highly represented than plasmacytoid DCs (pDCs). As in human skin, Langerhans cells (LCs) resided exclusively in the macaque epidermis, expressing CD11c, high levels of CD1a and langerin (CD207). Most DC subsets were endowed with tissue-specific combinations of PRRs. DCs generated from CD34(+) BM cells (CD34-DCs) were heterogeneous in phenotype. CD34-DCs shared properties (differentiation and PRR) of dermal and epidermal DCs. After injection into macaques, CD34-DCs expressing HIV-Gag induced Gag-specific CD4(+) and CD8(+) T cells producing IFN-γ, TNF-α, MIP-1β, or IL-2. In high responding animals, the numbers of polyfunctional CD8(+) T cells increased with the number of boo...Continue Reading

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Citations

May 13, 2014·Immunotherapy·Dige Andom Tesfatsion
Jul 30, 2015·Journal of Immunological Methods·Lucille AdamFrédéric Martinon
Oct 10, 2014·Clinical and Vaccine Immunology : CVI·Jason A SkinnerGerard Zurawski
Sep 26, 2015·PloS One·Anne-Laure FlamarYves Levy
May 10, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucille AdamFrédéric Martinon
Jul 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Olivier EpaulardFrédéric Martinon
Nov 4, 2017·Contrast Media & Molecular Imaging·Biliana TodorovaCatherine Chapon
Jul 3, 2021·Vaccines·Matthieu Van TilbeurghCaroline Manet

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