PMID: 14662335Dec 10, 2003Paper

CD34+CDw90(Thy-1)+ subset colocated with mesenchymal progenitors in human normal bone marrow hematon units is enriched in colony-forming unit megakaryocytes and long-term culture-initiating cells

Experimental Hematology
Nathalie BoiretMarc Gabriel Berger

Abstract

The progress made in the supportive care of allografts and the identification of mesenchymal stem cells in adult human bone marrow (BM) has prompted renewed interest in the use of BM as a form of cell therapy. With the aim of optimizing the collection of BM cells, we evaluated the hematopoietic and mesenchymal immature cell contents of BM hematon units (HUs), which usually are eliminated during graft processing. Hematopoietic CD34+ progenitors from HU and buffy coat (BC) compartments were characterized in short-term culture. The sorted CD34+CDw90(Thy-1)+ primitive subset was assessed in colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, then further characterized by the expression of additional antigens. In parallel, we evaluated the colony-forming unit fibroblast (CFU-F) number and phenotyped the fresh adherent (D1-3) cells. The plating efficiencies of CD34+ cells derived from HU and BC were identical. However, the HU CD34+CDw90(Thy-1)+ subset was enriched in colony-forming unit megakaryocyte (2.3x), LTC-IC (4.6x), and cells coexpressing CD105 (5x). We found a higher frequency of CFU-F (4.7x), considered to be the mesenchymal stem cell-containing population, correlated with an enrichment in fresh ...Continue Reading

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Citations

Sep 22, 2011·Stem Cells and Development·Carmen Mariana AaneiLydia Campos
Apr 24, 2010·Tissue Engineering. Part a·Darnell KaiglerWilliam V Giannobile
Jul 24, 2014·Stem Cells and Development·Alexandre JanelMarc G Berger
Jun 13, 2012·Cytotherapy·Naser AhmadbeigiNance Beyer Nardi
Mar 22, 2012·Advanced Drug Delivery Reviews·G PagniW V Giannobile
Oct 21, 2009·Journal of Cellular and Molecular Medicine·Frédéric DeschaseauxLuc Sensébé
Jan 29, 2005·Experimental Hematology·Nathalie BoiretMarc Gabriel Berger
Sep 22, 2007·The American Journal of Pathology·Katalin DezsoPeter Nagy
Jul 11, 2012·Cell Transplantation·Darnell KaiglerWilliam V Giannobile
Jun 24, 2017·American Journal of Hematology·Alexandre JanelMarc G Berger

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