CD36 and proteoglycan-mediated pathways for (n-3) fatty acid enriched triglyceride-rich particle blood clearance in mouse models in vivo and in peritoneal macrophages in vitro.

The Journal of Nutrition
Narumon DensupsoontornR J Deckelbaum

Abstract

Because the mechanisms of (n-3) fatty acid-enriched triglyceride-rich particle [(n-3)-TGRP] uptake are not well characterized, we questioned whether (n-3)-TGRP are removed via "nonclassical" pathways, e.g., pathways other than an LDL receptor and/or involving apolipoprotein E (apoE). Chylomicron-sized model (n-3)-TGRP labeled with [3H]cholesteryl ether were injected into wild-type (WT) and CD36 knockout (CD36-/-) mice at low, nonsaturating and high, saturating doses. Blood clearance of (n-3)-TGRP was determined by calculating fractional catabolic rates. At saturating doses, blood clearance of (n-3)-TGRP was slower in CD36-/- mice relative to WT mice, suggesting that in part CD36 contributes to (n-3)-TGRP uptake. To further examine the potential nonclassical clearance pathways, peritoneal-elicited macrophages from WT and CD36-/- mice were incubated with (n-3)-TGRP in the presence of apoE, lactoferrin, and/or sodium chlorate. Cellular (n-3)-TGRP uptake was measured to test the roles of apoE-mediated pathways and/or proteoglycans. ApoE-mediated pathways compensated in part for defective (n-3)-TGRP uptake in CD36-/- cells. Lactoferrin decreased (n-3)-TGRP uptake in the presence of apoE. Inhibition of cell proteoglycan synthesis by ...Continue Reading

References

Sep 10, 1994·Annals of the New York Academy of Sciences·R W MahleyD He
Jun 26, 1999·The Journal of Biological Chemistry·M FebbraioR L Silverstein
Oct 26, 2000·Current Opinion in Lipidology·R L Silverstein, M Febbraio
Sep 19, 2001·The Journal of Clinical Investigation·M FebbraioR L Silverstein
Jun 11, 2002·Annual Review of Nutrition·Tahar Hajri, Nada A Abumrad
Jan 1, 1965·The Journal of Experimental Medicine·Z A COHN, B BENSON
Feb 19, 2004·The Journal of Biological Chemistry·Ruud OutTheo J C Van Berkel
Aug 2, 2005·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Mimi N TonRichard J Deckelbaum
Mar 7, 2006·The Journal of Nutritional Biochemistry·Alfonso Alexander AguileraRosa M Oliart Ros

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Citations

Jan 11, 2011·Journal of Lipid Research·May BrundertFranz Rinninger
Jun 21, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Maria Febbraio
Feb 11, 2012·The Journal of Nutrition·Richard J Deckelbaum, Claudia Torrejon
Jan 9, 2010·Biochemical and Biophysical Research Communications·Faith M Murray-TaylorToru Seo

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